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Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes

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@article{f368b8ba9e3b41c58fafa6a90681dba3,
title = "Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes",
abstract = "The ability of bacteria to aggregate and form biofilms impairs phagocytosis by polymorphonuclear leukocytes (PMNs). The aim of this study was to examine if the size of aggregates is critical for successful phagocytosis and how bacterial biofilms evade phagocytosis. We investigated the live interaction between PMNs and Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Staphylococcus epidermidis using confocal scanning laser microscopy. Aggregate size significantly affected phagocytosis outcome and larger aggregates were less likely to be phagocytized. Aggregates of S. epidermidis were also less likely to be phagocytized than equally-sized aggregates of the other three species. We found that only aggregates of approx. 5 μm diameter or smaller were consistently phagocytosed. We demonstrate that planktonic and aggregated cells of all four species significantly reduced the viability of PMNs after 4 h of incubation. Our results indicate that larger bacterial aggregates are less likely to be phagocytosed by PMNs and we propose that, if the aggregates become too large, circulating PMNs may not be able to phagocytose them quickly enough, which may lead to chronic infection.",
keywords = "Biofilms, Escherichia coli/physiology, Humans, Neutrophils/physiology, Phagocytosis, Pseudomonas aeruginosa/physiology, Skin/microbiology, Staphylococcus aureus/physiology, Staphylococcus epidermidis/physiology, Polymorphonuclear leukocytes (PMNs), S. epidermidis, Chronic bacterial infection, S. aureus, E. coli, P. aeruginosa",
author = "Maria Alhede and Melanie Lorenz and Fritz, {Blaine Gabriel} and Jensen, {Peter {\O}strup} and Ring, {Hans Christian} and Lene Bay and Thomas Bjarnsholt",
year = "2020",
month = dec,
doi = "10.1007/s00430-020-00691-1",
language = "English",
volume = "209",
pages = "669--680",
journal = "Medical Microbiology and Immunology",
issn = "0300-8584",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes

AU - Alhede, Maria

AU - Lorenz, Melanie

AU - Fritz, Blaine Gabriel

AU - Jensen, Peter Østrup

AU - Ring, Hans Christian

AU - Bay, Lene

AU - Bjarnsholt, Thomas

PY - 2020/12

Y1 - 2020/12

N2 - The ability of bacteria to aggregate and form biofilms impairs phagocytosis by polymorphonuclear leukocytes (PMNs). The aim of this study was to examine if the size of aggregates is critical for successful phagocytosis and how bacterial biofilms evade phagocytosis. We investigated the live interaction between PMNs and Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Staphylococcus epidermidis using confocal scanning laser microscopy. Aggregate size significantly affected phagocytosis outcome and larger aggregates were less likely to be phagocytized. Aggregates of S. epidermidis were also less likely to be phagocytized than equally-sized aggregates of the other three species. We found that only aggregates of approx. 5 μm diameter or smaller were consistently phagocytosed. We demonstrate that planktonic and aggregated cells of all four species significantly reduced the viability of PMNs after 4 h of incubation. Our results indicate that larger bacterial aggregates are less likely to be phagocytosed by PMNs and we propose that, if the aggregates become too large, circulating PMNs may not be able to phagocytose them quickly enough, which may lead to chronic infection.

AB - The ability of bacteria to aggregate and form biofilms impairs phagocytosis by polymorphonuclear leukocytes (PMNs). The aim of this study was to examine if the size of aggregates is critical for successful phagocytosis and how bacterial biofilms evade phagocytosis. We investigated the live interaction between PMNs and Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Staphylococcus epidermidis using confocal scanning laser microscopy. Aggregate size significantly affected phagocytosis outcome and larger aggregates were less likely to be phagocytized. Aggregates of S. epidermidis were also less likely to be phagocytized than equally-sized aggregates of the other three species. We found that only aggregates of approx. 5 μm diameter or smaller were consistently phagocytosed. We demonstrate that planktonic and aggregated cells of all four species significantly reduced the viability of PMNs after 4 h of incubation. Our results indicate that larger bacterial aggregates are less likely to be phagocytosed by PMNs and we propose that, if the aggregates become too large, circulating PMNs may not be able to phagocytose them quickly enough, which may lead to chronic infection.

KW - Biofilms

KW - Escherichia coli/physiology

KW - Humans

KW - Neutrophils/physiology

KW - Phagocytosis

KW - Pseudomonas aeruginosa/physiology

KW - Skin/microbiology

KW - Staphylococcus aureus/physiology

KW - Staphylococcus epidermidis/physiology

KW - Polymorphonuclear leukocytes (PMNs)

KW - S. epidermidis

KW - Chronic bacterial infection

KW - S. aureus

KW - E. coli

KW - P. aeruginosa

UR - http://www.scopus.com/inward/record.url?scp=85090088733&partnerID=8YFLogxK

U2 - 10.1007/s00430-020-00691-1

DO - 10.1007/s00430-020-00691-1

M3 - Journal article

C2 - 32880037

VL - 209

SP - 669

EP - 680

JO - Medical Microbiology and Immunology

JF - Medical Microbiology and Immunology

SN - 0300-8584

IS - 6

ER -

ID: 61840935