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Region Hovedstaden - en del af Københavns Universitetshospital
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Autofluorescence in samples obtained from chronic biofilm infections - "all that glitters is not gold"

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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Vis graf over relationer

When looking at tissue sections of ex vivo samples, autofluorescence can be a major cause of artifacts and misinterpretations. We here reiterate evidence that autofluorescing granules, often hemosiderin but also ceroid or mucinogen granules, are severe obstacles when imaging and diagnosing biofilm infections through fluorescent imaging techniques. We used confocal laser scanning microscopy with spectral analysis for autofluorescence detection as well as standard histological stains in order to identify the culprit and show that these granules might very well be mistaken for bacterial biofilms. Furthermore, we hypothesize that the increased amount of autofluorescing granules may be a consequence of prolonged inflammation as a consequence of chronic biofilm infections.

OriginalsprogEngelsk
TidsskriftPathogens and Disease
Vol/bind73
Udgave nummer4
Sider (fra-til)pii
Antal sider4
DOI
StatusUdgivet - jun. 2015

ID: 45317653