TY - JOUR
T1 - Association of Chlamydia pneumoniae with coronary artery disease and its progression is dependent on the modifying effect of mannose-binding lectin
AU - Rugonfalvi-Kiss, Szabolcs
AU - Endrész, Valéria
AU - Madsen, Hans O
AU - Burián, Katalin
AU - Duba, Jenô
AU - Prohászka, Zoltán
AU - Karádi, István
AU - Romics, László
AU - Gönczöl, Eva
AU - Füst, George
AU - Garred, Peter
PY - 2002/8/27
Y1 - 2002/8/27
N2 - BACKGROUND: The possible association between coronary artery disease (CAD) and Chlamydia pneumoniae (C pneumoniae) infection is controversial. On the basis of the recent suggestion that mannose-binding lectin (MBL) variant alleles are related to an increased risk of severe atherosclerosis, and on the in vitro interaction of MBL with C pneumoniae, we asked whether MBL might contribute to CAD in conjunction with C pneumoniae.METHODS AND RESULTS: Antibodies to C pneumoniae were measured by immunofluorescence and MBL alleles were determined by polymerase chain reaction technique in samples from 210 patients with CAD and 257 healthy subjects from Hungary collected between 1995 and 1996. A higher percentage of patients with CAD were anti-C pneumoniae positive as compared with the control group (P=0.058). However, at logistic regression analysis adjusted to age, sex, and serum lipid levels, this difference was confined only to subjects carrying MBL variant alleles (P=0.035, odds ratio 2.63, [95% CI: 1.07 to 6.45]). In contrast, no significant difference was seen in those homozygous for the normal MBL allele (P=0.412). During a 65+/-5.8-month follow-up period, major outcomes (new myocardial infarction, and/or bypass operation or cardiovascular death) occurred in 11 C pneumoniae positive and 3 C pneumoniae negative patients. In the C pneumoniae positive group, the odds ratio of development of outcomes was 3.27 (95% CI: 1.10 to 9.71, P=0.033) in the carriers of the MBL variant alleles compared with the homozygous carriers of the normal MBL allele.CONCLUSIONS: These results indicate that infection with C pneumoniae leads mainly to the development and progression of severe CAD in patients with variation in the MBL gene.
AB - BACKGROUND: The possible association between coronary artery disease (CAD) and Chlamydia pneumoniae (C pneumoniae) infection is controversial. On the basis of the recent suggestion that mannose-binding lectin (MBL) variant alleles are related to an increased risk of severe atherosclerosis, and on the in vitro interaction of MBL with C pneumoniae, we asked whether MBL might contribute to CAD in conjunction with C pneumoniae.METHODS AND RESULTS: Antibodies to C pneumoniae were measured by immunofluorescence and MBL alleles were determined by polymerase chain reaction technique in samples from 210 patients with CAD and 257 healthy subjects from Hungary collected between 1995 and 1996. A higher percentage of patients with CAD were anti-C pneumoniae positive as compared with the control group (P=0.058). However, at logistic regression analysis adjusted to age, sex, and serum lipid levels, this difference was confined only to subjects carrying MBL variant alleles (P=0.035, odds ratio 2.63, [95% CI: 1.07 to 6.45]). In contrast, no significant difference was seen in those homozygous for the normal MBL allele (P=0.412). During a 65+/-5.8-month follow-up period, major outcomes (new myocardial infarction, and/or bypass operation or cardiovascular death) occurred in 11 C pneumoniae positive and 3 C pneumoniae negative patients. In the C pneumoniae positive group, the odds ratio of development of outcomes was 3.27 (95% CI: 1.10 to 9.71, P=0.033) in the carriers of the MBL variant alleles compared with the homozygous carriers of the normal MBL allele.CONCLUSIONS: These results indicate that infection with C pneumoniae leads mainly to the development and progression of severe CAD in patients with variation in the MBL gene.
KW - Alleles
KW - Antibodies, Bacterial/blood
KW - C-Reactive Protein/analysis
KW - Carrier Proteins/genetics
KW - Chaperonin 60/immunology
KW - Chlamydophila Infections/blood
KW - Chlamydophila pneumoniae/immunology
KW - Collectins
KW - Comorbidity
KW - Coronary Artery Disease/blood
KW - Demography
KW - Disease Progression
KW - Female
KW - Gene Frequency
KW - Genetic Carrier Screening
KW - Genetic Variation
KW - Humans
KW - Hungary/epidemiology
KW - Immunoglobulin G/blood
KW - Logistic Models
KW - Male
KW - Middle Aged
KW - Odds Ratio
KW - Polymorphism, Genetic
KW - Predictive Value of Tests
KW - Risk Factors
KW - Seroepidemiologic Studies
U2 - 10.1161/01.cir.0000027137.96791.6a
DO - 10.1161/01.cir.0000027137.96791.6a
M3 - Journal article
C2 - 12196331
SN - 1524-4539
VL - 106
SP - 1071
EP - 1076
JO - Circulation
JF - Circulation
IS - 9
ER -