Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

Dres Damgaard; Claus H Nielsen

doi:10.1007/978-1-4939-8949-2_20

Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

Dres Damgaard
, Claus H Nielsen

Afdeling for Rygkirurgi, Led- og Bindevævssygdomme HOC

1 Citationer (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) for measurement of the activity of peptidylarginine deiminases (PADs), the enzymes responsible for citrullination, is described. It uses fibrinogen as substrate for the enzyme and a commercial antibody specific for the citrullinated form of fibrinogen.

Originalsprog	Engelsk
Tidsskrift	Methods in molecular biology
Vol/bind	1901
Sider (fra-til)	239-242
Antal sider	4
ISSN	1064-3745
DOI	https://doi.org/10.1007/978-1-4939-8949-2_20
Status	Udgivet - 2019

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10.1007/978-1-4939-8949-2_20

Citationsformater

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title = "Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate",

abstract = "An enzyme-linked immunosorbent assay (ELISA) for measurement of the activity of peptidylarginine deiminases (PADs), the enzymes responsible for citrullination, is described. It uses fibrinogen as substrate for the enzyme and a commercial antibody specific for the citrullinated form of fibrinogen.",

keywords = "Enzyme Assays, Enzyme-Linked Immunosorbent Assay/methods, Fibrinogen/metabolism, Humans, Protein-Arginine Deiminases/metabolism, Substrate Specificity",

author = "Dres Damgaard and Nielsen, \{Claus H\}",

year = "2019",

doi = "10.1007/978-1-4939-8949-2\_20",

language = "English",

volume = "1901",

pages = "239--242",

journal = "Methods in molecular biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

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TY - JOUR

T1 - Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

AU - Damgaard, Dres

AU - Nielsen, Claus H

PY - 2019

Y1 - 2019

N2 - An enzyme-linked immunosorbent assay (ELISA) for measurement of the activity of peptidylarginine deiminases (PADs), the enzymes responsible for citrullination, is described. It uses fibrinogen as substrate for the enzyme and a commercial antibody specific for the citrullinated form of fibrinogen.

AB - An enzyme-linked immunosorbent assay (ELISA) for measurement of the activity of peptidylarginine deiminases (PADs), the enzymes responsible for citrullination, is described. It uses fibrinogen as substrate for the enzyme and a commercial antibody specific for the citrullinated form of fibrinogen.

KW - Enzyme Assays

KW - Enzyme-Linked Immunosorbent Assay/methods

KW - Fibrinogen/metabolism

KW - Humans

KW - Protein-Arginine Deiminases/metabolism

KW - Substrate Specificity

U2 - 10.1007/978-1-4939-8949-2_20

DO - 10.1007/978-1-4939-8949-2_20

M3 - Journal article

C2 - 30539583

SN - 1064-3745

VL - 1901

SP - 239

EP - 242

JO - Methods in molecular biology

JF - Methods in molecular biology

ER -

Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

Abstract

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