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Assay of mast cell mediators

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  1. Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance

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  2. Analysis of Mass Cytometry Data

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  3. Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

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  4. Full-Length Open Reading Frame Amplification of Hepatitis C Virus

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  5. In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

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  1. Rational Design, Structure-Activity Relationship, and Immunogenicity of Hypoallergenic Pru p 3 Variants

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. No difference in human mast cells derived from peanut allergic versus non-allergic subjects

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  3. Beyond IgE-When Do IgE-Crosslinking and Effector Cell Activation Lead to Clinical Anaphylaxis?

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  4. Bioactive small molecules in commercially available cereal food: Benzoxazinoids

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Vis graf over relationer

Mediator release from activated mast cells is a major initiator of the symptomology associated with allergic disorders such as anaphylaxis and asthma. Thus, methods to monitor the generation and release of such mediators have widespread applicability in studies designed to understand the processes regulating mast cell activation and for the identification of therapeutic approaches to block mast cell-driven disease. In this chapter, we discuss approaches used for the determination of mast cell degranulation, lipid-derived inflammatory mediator production, and cytokine/chemokine gene expression as well as cytokine release.

OriginalsprogEngelsk
TidsskriftMethods in Molecular Biology
Vol/bind1220
Sider (fra-til)307-23
Antal sider17
ISSN1064-3745
DOI
StatusUdgivet - 2015

ID: 44773705