Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses

Kathryn Rozen-Gagnon, Meigang Gu, Joseph M Luna, Ji-Dung Luo, Soon Yi, Sasha Novack, Eliana Jacobson, Wei Wang, Matthew R Paul, Troels K H Scheel, Thomas Carroll, Charles M Rice

4 Citationer (Scopus)

Abstract

Argonaute (AGO) proteins bind small RNAs to silence complementary RNA transcripts, and they are central to RNA interference (RNAi). RNAi is critical for regulation of gene expression and antiviral defense in Aedes aegypti mosquitoes, which transmit Zika, chikungunya, dengue, and yellow fever viruses. In mosquitoes, AGO1 mediates miRNA interactions, while AGO2 mediates siRNA interactions. We applied AGO-crosslinking immunoprecipitation (AGO-CLIP) for both AGO1 and AGO2, and we developed a universal software package for CLIP analysis (CLIPflexR), identifying 230 small RNAs and 5,447 small RNA targets that comprise a comprehensive RNAi network map in mosquitoes. RNAi network maps predicted expression levels of small RNA targets in specific tissues. Additionally, this resource identified unexpected, context-dependent AGO2 target preferences, including endogenous viral elements and 3'UTRs. Finally, contrary to current thinking, mosquito AGO2 repressed imperfect targets. These findings expand our understanding of small RNA networks and have broad implications for the study of antiviral RNAi.

OriginalsprogEngelsk
Artikelnummere13
TidsskriftCell Host & Microbe
Vol/bind29
Udgave nummer5
Sider (fra-til)834-848
Antal sider14
ISSN1931-3128
DOI
StatusUdgivet - 12 maj 2021

Fingeraftryk

Dyk ned i forskningsemnerne om 'Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses'. Sammen danner de et unikt fingeraftryk.

Citationsformater