Abstract
Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically defined cell populations with subsequent analyses of leukemia-associated cytogenetic and molecular marker. The approaches described here optimize the use of the same tube of unfixed, antibody-stained BM cells for flow-sorting of small cell populations and subsequent exploratory FISH and PCR-based analyses.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Immunological Methods |
Vol/bind | 369 |
Udgave nummer | 1-2 |
Sider (fra-til) | 69-73 |
Antal sider | 5 |
ISSN | 0022-1759 |
DOI | |
Status | Udgivet - 2011 |