Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

Apolipoprotein M-bound sphingosine-1-phosphate regulates blood-brain barrier paracellular permeability and transcytosis

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Soluble collectin-12 mediates C3-independent docking of properdin that activates the alternative pathway of complement

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Quantification of the pace of biological aging in humans through a blood test, the DunedinPoAm DNA methylation algorithm

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Body height in young adult men and risk of dementia later in adult life

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Linking glycemic dysregulation in diabetes to symptoms, comorbidities, and genetics through EHR data mining

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Modification of oxygen consumption and blood flow in mouse somatosensory cortex by cell-type-specific neuronal activity

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Vascular function in adults with cyanotic congenital heart disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Apolipoprotein M and risk of type 2 diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Effect of insulin on natriuretic peptide gene expression in porcine heart

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

The blood-brain barrier (BBB) is formed by the endothelial cells lining cerebral microvessels, but how blood-borne signaling molecules influence permeability is incompletely understood. We here examined how the apolipoprotein M (apoM)-bound sphingosine 1-phosphate (S1P) signaling pathway affects the BBB in different categories of cerebral microvessels using ApoM deficient mice (Apom-/-). We used two-photon microscopy to monitor BBB permeability of sodium fluorescein (376 Da), Alexa Fluor (643 Da), and fluorescent albumin (45 kDA). We show that BBB permeability to small molecules increases in Apom-/- mice. Vesicle-mediated transfer of albumin in arterioles increased 3 to 10-fold in Apom-/- mice, whereas transcytosis in capillaries and venules remained unchanged. The S1P receptor 1 agonist SEW2871 rapidly normalized paracellular BBB permeability in Apom-/- mice, and inhibited transcytosis in penetrating arterioles, but not in pial arterioles. Thus, apoM-bound S1P maintains low paracellular BBB permeability in all cerebral microvessels and low levels of vesicle-mediated transport in penetrating arterioles.

OriginalsprogEngelsk
Artikelnummere49405
TidsskrifteLife
Vol/bind8
ISSN2050-084X
DOI
StatusUdgivet - 25 nov. 2019

Bibliografisk note

© 2019, Mathiesen Janiurek et al.

ID: 58504999