Abstract
We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD-women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th-36th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.
Bidragets oversatte titel | Antenatal determination of fetal RhD-blood type based on fetal DNA in plasma from the RhD-negative mother--secondary publication |
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Originalsprog | Dansk |
Tidsskrift | Ugeskrift for Laeger |
Vol/bind | 168 |
Udgave nummer | 26-32 |
Sider (fra-til) | 2568-70 |
Antal sider | 3 |
ISSN | 0041-5782 |
Status | Udgivet - 26 jun. 2006 |
Emneord
- DNA
- DNA Probes
- Exons
- Female
- Fetal Diseases
- Genotype
- Humans
- Polymerase Chain Reaction
- Pregnancy
- Prenatal Diagnosis
- Rh-Hr Blood-Group System
- Sensitivity and Specificity