Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
E-pub ahead of print

Analysis of the organization and expression patterns of the convergent Pseudomonas aeruginosa lasR/rsaL gene pair uncovers mutual influence

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Targeting bioenergetics is key to counteracting the drug-tolerant state of biofilm-grown bacteria

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Mining zebrafish microbiota reveals key community-level resistance against fish pathogen infection

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Host-induced spermidine production in motile Pseudomonas aeruginosa triggers phagocytic uptake

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

The two adjacent genes encoding the major Pseudomonas aeruginosa quorum-sensing regulator, LasR, and its opponent, RsaL, overlap in their coding 3' ends and produce mRNA transcripts with long untranslated 3' ends that overlap with the sense transcripts of the gene on the opposing DNA strand. In this study, we evaluated whether the overlapping genes are involved in mutual regulatory events and studied interference by natural antisense transcripts. We introduced various gene expression constructs into a P. aeruginosa PA14 lasR/rsaL double deletion mutant, and found that although complementary RNA is produced, this does not interfere with the sense gene expression levels of lasR and rsaL and does not have functional consequences on down-stream gene regulation. Nevertheless, expression of lasR, but not of rsaL, was shown to be enhanced if transcription was terminated at the end of the respective gene so that no overlapping transcription was allowed. Our data indicate that the natural organization with a partial overlap at the 3' ends of the lasR/rsaL genes gives rise to a system of checks and balances to prevent dominant and unilateral control by LasR over the RsaL transcriptional regulator of opposing function.

OriginalsprogEngelsk
TidsskriftMolecular Microbiology
ISSN0950-382X
DOI
StatusE-pub ahead of print - 19 okt. 2021

Bibliografisk note

© 2020 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

ID: 62335727