Analysis of epigenetic modifications of DNA in human cells

Lasse Sommer Kristensen; Marianne Bach Treppendahl; Kirsten Grønbæk

doi:10.1002/0471142905.hg2002s77

Analysis of epigenetic modifications of DNA in human cells

Lasse Sommer Kristensen, Marianne Bach Treppendahl, Kirsten Grønbæk

15 Citationer (Scopus)

Abstract

Epigenetics, the study of somatically heritable changes in gene expression not related to changes in the DNA sequence, is a rapidly expanding research field that plays important roles in healthy as well as in diseased cells. DNA methylation and hydroxymethylation are epigenetic modifications found in human cells, which are deeply implicated in normal cellular processes as well as in several major human diseases. Here, a range of different methods for the analyses of DNA methylation and hydroxymethylation at locus-specific and genome-wide scales is described.

Originalsprog	Engelsk
Tidsskrift	Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
Vol/bind	Chapter 20
Sider (fra-til)	Unit20.2
DOI	https://doi.org/10.1002/0471142905.hg2002s77
Status	Udgivet - apr. 2013

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10.1002/0471142905.hg2002s77

Citationsformater

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title = "Analysis of epigenetic modifications of DNA in human cells",

abstract = "Epigenetics, the study of somatically heritable changes in gene expression not related to changes in the DNA sequence, is a rapidly expanding research field that plays important roles in healthy as well as in diseased cells. DNA methylation and hydroxymethylation are epigenetic modifications found in human cells, which are deeply implicated in normal cellular processes as well as in several major human diseases. Here, a range of different methods for the analyses of DNA methylation and hydroxymethylation at locus-specific and genome-wide scales is described.",

keywords = "Cell Transformation, Neoplastic, DNA, DNA Methylation, DNA Primers, Epigenesis, Genetic, Eukaryotic Cells, Gene Expression, Genome, Humans, Polymerase Chain Reaction, Sequence Analysis, DNA",

author = "Kristensen, {Lasse Sommer} and Treppendahl, {Marianne Bach} and Kirsten Gr{\o}nb{\ae}k",

year = "2013",

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doi = "10.1002/0471142905.hg2002s77",

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TY - JOUR

T1 - Analysis of epigenetic modifications of DNA in human cells

AU - Kristensen, Lasse Sommer

AU - Treppendahl, Marianne Bach

AU - Grønbæk, Kirsten

PY - 2013/4

Y1 - 2013/4

N2 - Epigenetics, the study of somatically heritable changes in gene expression not related to changes in the DNA sequence, is a rapidly expanding research field that plays important roles in healthy as well as in diseased cells. DNA methylation and hydroxymethylation are epigenetic modifications found in human cells, which are deeply implicated in normal cellular processes as well as in several major human diseases. Here, a range of different methods for the analyses of DNA methylation and hydroxymethylation at locus-specific and genome-wide scales is described.

AB - Epigenetics, the study of somatically heritable changes in gene expression not related to changes in the DNA sequence, is a rapidly expanding research field that plays important roles in healthy as well as in diseased cells. DNA methylation and hydroxymethylation are epigenetic modifications found in human cells, which are deeply implicated in normal cellular processes as well as in several major human diseases. Here, a range of different methods for the analyses of DNA methylation and hydroxymethylation at locus-specific and genome-wide scales is described.

KW - Cell Transformation, Neoplastic

KW - DNA

KW - DNA Methylation

KW - DNA Primers

KW - Epigenesis, Genetic

KW - Eukaryotic Cells

KW - Gene Expression

KW - Genome

KW - Humans

KW - Polymerase Chain Reaction

KW - Sequence Analysis, DNA

U2 - 10.1002/0471142905.hg2002s77

DO - 10.1002/0471142905.hg2002s77

M3 - Journal article

C2 - 23595599

SN - 1934-8258

VL - Chapter 20

SP - Unit20.2

JO - Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]

JF - Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]

ER -

Analysis of epigenetic modifications of DNA in human cells

Abstract

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