An Optimized Mouse Brain Atlas for Automated Mapping and Quantification of Neuronal Activity Using iDISCO+ and Light Sheet Fluorescence Microscopy

Johanna Perens, Casper Gravesen Salinas, Jacob Lercke Skytte, Urmas Roostalu, Anders Bjorholm Dahl, Tim B Dyrby, Franziska Wichern, Pernille Barkholt, Niels Vrang, Jacob Jelsing, Jacob Hecksher-Sørensen

16 Citationer (Scopus)

Abstrakt

In recent years, the combination of whole-brain immunolabelling, light sheet fluorescence microscopy (LSFM) and subsequent registration of data with a common reference atlas, has enabled 3D visualization and quantification of fluorescent markers or tracers in the adult mouse brain. Today, the common coordinate framework version 3 developed by the Allen's Institute of Brain Science (AIBS CCFv3), is widely used as the standard brain atlas for registration of LSFM data. However, the AIBS CCFv3 is based on histological processing and imaging modalities different from those used for LSFM imaging and consequently, the data differ in both tissue contrast and morphology. To improve the accuracy and speed by which LSFM-imaged whole-brain data can be registered and quantified, we have created an optimized digital mouse brain atlas based on immunolabelled and solvent-cleared brains. Compared to the AIBS CCFv3 atlas, our atlas resulted in faster and more accurate mapping of neuronal activity as measured by c-Fos expression, especially in the hindbrain. We further demonstrated utility of the LSFM atlas by comparing whole-brain quantitative changes in c-Fos expression following acute administration of semaglutide in lean and diet-induced obese mice. In combination with an improved algorithm for c-Fos detection, the LSFM atlas enables unbiased and computationally efficient characterization of drug effects on whole-brain neuronal activity patterns. In conclusion, we established an optimized reference atlas for more precise mapping of fluorescent markers, including c-Fos, in mouse brains processed for LSFM.

OriginalsprogEngelsk
TidsskriftNeuroinformatics
Vol/bind19
Udgave nummer3
Sider (fra-til)433-446
Antal sider14
ISSN1539-2791
DOI
StatusUdgivet - jul. 2021

Fingeraftryk

Dyk ned i forskningsemnerne om 'An Optimized Mouse Brain Atlas for Automated Mapping and Quantification of Neuronal Activity Using iDISCO+ and Light Sheet Fluorescence Microscopy'. Sammen danner de et unikt fingeraftryk.

Citationsformater