An inactivated SARS-CoV-2 vaccine induced cross-neutralizing persisting antibodies and protected against challenge in small animals

Anna Offersgaard, Carlos Rene Duarte Hernandez, Shan Feng, Pavel Marichal-Gallardo, Kenn Holmbeck, Anne Finne Pihl, Carlota Fernandez-Antunez, Garazi Peña Alzua, Katrine Top Hartmann, Long V Pham, Yuyong Zhou, Karen Anbro Gammeltoft, Ulrik Fahnøe, Uffe Vest Schneider, Gabriel Kristian Pedersen, Henrik Elvang Jensen, Jan Pravsgaard Christensen, Santseharay Ramirez, Jens Bukh, Judith Margarete Gottwein

Abstract

Vaccines have relieved the public health burden of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and globally inactivated vaccines are most widely used. However, poor vaccination accessibility and waning immunity maintain the pandemic, driving emergence of variants. We developed an inactivated SARS-CoV-2 (I-SARS-CoV-2) vaccine based on a viral isolate with the Spike mutation D614G, produced in Vero cells in a scalable bioreactor, inactivated with β-propiolactone, purified by membrane-based steric exclusion chromatography, and adjuvanted with MF59-like adjuvant AddaVax. I-SARS-CoV-2 and a derived split vaccine induced persisting neutralizing antibodies in mice; moreover, lyophilized antigen was immunogenic. Following homologous challenge, I-SARS-CoV-2 immunized hamsters were protected against disease and lung pathology. In contrast with reports for widely used vaccines, hamster plasma similarly neutralized the homologous and the Delta (B.1.617.2) variant viruses, whereas the Omicron (B.1.1.529) variant was neutralized less efficiently. Applied bioprocessing approaches offer advantages regarding scalability and production, potentially benefitting worldwide vaccine coverage.

OriginalsprogEngelsk
Artikelnummer105949
TidsskriftiScience
Vol/bind26
Udgave nummer2
Sider (fra-til)105949
ISSN2589-0042
DOI
StatusUdgivet - 17 feb. 2023

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