Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

An in vivo Mouse Model to Investigate the Effect of Local Anesthetic Nanomedicines on Axonal Conduction and Excitability

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Microstates as Disease and Progression Markers in Patients With Mild Cognitive Impairment

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. A Comparison of Regularization Methods in Forward and Backward Models for Auditory Attention Decoding

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Computing Generalized Matrix Inverse on Spiking Neural Substrate

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Deep Learning Based Attenuation Correction of PET/MRI in Pediatric Brain Tumor Patients: Evaluation in a Clinical Setting

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Adductor canal block with a suture-method catheter - A parallel or perpendicular approach?

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Neurologiske Sygdomme

    Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiUndervisningpeer review

  3. Local Anesthetic Injection Speed and Common Peroneal Nerve Block Duration: A Randomized Controlled Trial in Healthy Volunteers

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Peripheral nerve blocks (PNBs) using local anesthetic (LA) are superior to systemic analgesia for management of post-operative pain. An insufficiently short PNB duration following single-shot LA can be optimized by development of extended release formulations among which liposomes have been shown to be the least toxic. In vivo rodent models for PNB have focused primarily on assessing behavioral responses following LA. In a previous study in human volunteers, we found that it is feasible to monitor the effect of LA in vivo by combining conventional conduction studies with nerve excitability studies. Here, we aimed to develop a mouse model where the same neurophysiological techniques can be used to investigate liposomal formulations of LA in vivo. To challenge the validity of the model, we tested the motor PNB following an unilamellar liposomal formulation, filled with the intermediate-duration LA lidocaine. Experiments were carried out in adult transgenic mice with fluorescent axons and with fluorescent tagged liposomes to allow in vivo imaging by probe-based confocal laser endomicroscopy. Recovery of conduction following LA injection at the ankle was monitored by stimulation of the tibial nerve fibers at the sciatic notch and recording of the plantar compound motor action potential (CMAP). We detected a delayed recovery in CMAP amplitude following liposomal lidocaine, without detrimental systemic effects. Furthermore, CMAP threshold-tracking studies of the distal tibial nerve showed that the increased rheobase was associated with a sequence of excitability changes similar to those found following non-encapsulated lidocaine PNB in humans, further supporting the translational value of the model.

OriginalsprogEngelsk
TidsskriftFrontiers in Neuroscience
Vol/bind12
Sider (fra-til)494
ISSN1662-4548
DOI
StatusUdgivet - 2018

ID: 54977883