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Allele-Specific DNA Methylation Detection by Pyrosequencing®

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Vis graf over relationer

DNA methylation is an epigenetic modification that plays important roles in healthy as well as diseased cells, by influencing the transcription of genes. In spite the fact that human somatic cells are diploid, most of the currently available methods for the study of DNA methylation do not provide information on the methylation status of individual alleles of genes. This information may be of importance in many situations. In particular, in cancer both alleles of tumour suppressor genes generally need to be inactivated for a phenotypic effect to be observed. Here, we present a simple and cost-effective protocol for allele-specific DNA methylation detection based on Pyrosequencing(®) of methylation-specific PCR (MSP) products including a single nucleotide polymorphism (SNP) within the amplicon.

OriginalsprogEngelsk
TidsskriftMethods in molecular biology
Vol/bind1315
Sider (fra-til)271-89
Antal sider19
ISSN1064-3745
DOI
StatusUdgivet - 2015

ID: 46176966