TY - JOUR
T1 - Advanced microtechnologies for detection of chromosome abnormalities by fluorescent in situ hybridization
AU - Kwasny, Dorota
AU - Vedarethinam, Indumathi
AU - Shah, Pranjul Jaykumar
AU - Dimaki, Maria
AU - Silahtaroglu, Asli
AU - Tumer, Zeynep
AU - Svendsen, Winnie Edith
PY - 2012
Y1 - 2012
N2 - Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time. The recent focus on the development of automated systems for performing FISH on chip is summarized in this review.
AB - Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time. The recent focus on the development of automated systems for performing FISH on chip is summarized in this review.
KW - Bioreactors
KW - Cell Culture Techniques
KW - Chromosome Aberrations
KW - Equipment Design
KW - Humans
KW - In Situ Hybridization, Fluorescence
KW - Metaphase
KW - Microfluidics
U2 - 10.1007/s10544-011-9622-7
DO - 10.1007/s10544-011-9622-7
M3 - Journal article
C2 - 22222279
SN - 1387-2176
VL - 14
SP - 453
EP - 460
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 3
ER -