TY - CHAP
T1 - Adipogenesis in Primary Cell Culture
AU - Larsen, Therese Juhlin
AU - Jespersen, Naja Zenius
AU - Scheele, Camilla
PY - 2019
Y1 - 2019
N2 - Obesity involves a contrasting expansion of the energy-storing white fat and loss of functionally competent brown fat, an energy-consuming thermogenic adipose. Leveraging our understanding of white and brown adipocyte recruitment and investigating factors that regulate these processes might reveal novel targets for counteracting obesity. In vitro differentiation of primary preadipocytes mimics many of the morphological and transcriptional events occurring during adipogenesis in vivo. Moreover, preadipocytes isolated from a specific depot maintain features of their originating niche. This makes in vitro adipogenesis a valuable model for identifying differential regulation patterns between brown and white adipogenesis. In this chapter, we describe step-by-step how to isolate brown and white preadipocytes from human tissue biopsies and how to culture and differentiate them in vitro. We discuss this process, what to consider, and how this in vitro system can be used to model in vivo adipogenesis.
AB - Obesity involves a contrasting expansion of the energy-storing white fat and loss of functionally competent brown fat, an energy-consuming thermogenic adipose. Leveraging our understanding of white and brown adipocyte recruitment and investigating factors that regulate these processes might reveal novel targets for counteracting obesity. In vitro differentiation of primary preadipocytes mimics many of the morphological and transcriptional events occurring during adipogenesis in vivo. Moreover, preadipocytes isolated from a specific depot maintain features of their originating niche. This makes in vitro adipogenesis a valuable model for identifying differential regulation patterns between brown and white adipogenesis. In this chapter, we describe step-by-step how to isolate brown and white preadipocytes from human tissue biopsies and how to culture and differentiate them in vitro. We discuss this process, what to consider, and how this in vitro system can be used to model in vivo adipogenesis.
KW - Adipogenesis
KW - Brown fat
KW - Human brown adipocytes
KW - In vitro differentiation
KW - Obesity
KW - White fat
KW - Humans
KW - Adipose Tissue, Brown/metabolism
KW - Adipocytes, Brown
KW - Cell Differentiation
KW - Primary Cell Culture
KW - Adipose Tissue, White/metabolism
UR - http://www.scopus.com/inward/record.url?scp=85064193105&partnerID=8YFLogxK
U2 - 10.1007/164_2018_142
DO - 10.1007/164_2018_142
M3 - Book chapter
C2 - 29980911
VL - 251
T3 - Handbook of Experimental Pharmacology
SP - 73
EP - 84
BT - Handbook of Experimental Pharmacology
ER -