Adipogenesis in Primary Cell Culture

Therese Juhlin Larsen, Naja Zenius Jespersen, Camilla Scheele

6 Citationer (Scopus)

Abstract

Obesity involves a contrasting expansion of the energy-storing white fat and loss of functionally competent brown fat, an energy-consuming thermogenic adipose. Leveraging our understanding of white and brown adipocyte recruitment and investigating factors that regulate these processes might reveal novel targets for counteracting obesity. In vitro differentiation of primary preadipocytes mimics many of the morphological and transcriptional events occurring during adipogenesis in vivo. Moreover, preadipocytes isolated from a specific depot maintain features of their originating niche. This makes in vitro adipogenesis a valuable model for identifying differential regulation patterns between brown and white adipogenesis. In this chapter, we describe step-by-step how to isolate brown and white preadipocytes from human tissue biopsies and how to culture and differentiate them in vitro. We discuss this process, what to consider, and how this in vitro system can be used to model in vivo adipogenesis.

OriginalsprogEngelsk
TitelHandbook of Experimental Pharmacology
Antal sider12
Vol/bind251
Publikationsdato2019
Sider73-84
DOI
StatusUdgivet - 2019
NavnHandbook of Experimental Pharmacology
ISSN0171-2004

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