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Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients

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Harvard

Candido Caçador, N, Paulino da Costa Capizzani, C, Gomes Monteiro Marin Torres, LA, Galetti, R, Ciofu, O, da Costa Darini, AL & Høiby, N 2018, 'Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients' PLoS One, bind 13, nr. 11, s. e0208013. https://doi.org/10.1371/journal.pone.0208013

APA

Candido Caçador, N., Paulino da Costa Capizzani, C., Gomes Monteiro Marin Torres, L. A., Galetti, R., Ciofu, O., da Costa Darini, A. L., & Høiby, N. (2018). Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients. PLoS One, 13(11), e0208013. https://doi.org/10.1371/journal.pone.0208013

CBE

Candido Caçador N, Paulino da Costa Capizzani C, Gomes Monteiro Marin Torres LA, Galetti R, Ciofu O, da Costa Darini AL, Høiby N. 2018. Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients. PLoS One. 13(11):e0208013. https://doi.org/10.1371/journal.pone.0208013

MLA

Vancouver

Candido Caçador N, Paulino da Costa Capizzani C, Gomes Monteiro Marin Torres LA, Galetti R, Ciofu O, da Costa Darini AL o.a. Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients. PLoS One. 2018;13(11):e0208013. https://doi.org/10.1371/journal.pone.0208013

Author

Candido Caçador, Natália ; Paulino da Costa Capizzani, Carolina ; Gomes Monteiro Marin Torres, Lídia Alice ; Galetti, Renata ; Ciofu, Oana ; da Costa Darini, Ana Lúcia ; Høiby, Niels. / Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients. I: PLoS One. 2018 ; Bind 13, Nr. 11. s. e0208013.

Bibtex

@article{96c13a717ff14d478840b5b43b346d98,
title = "Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients",
abstract = "Chronic lung infection by Pseudomonas aeruginosa is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. This is associated with the conversion of the non-mucoid to the mucoid phenotype. However, there is little information about the occurrence of alginate-producing P. aeruginosa in CF patients outside Europe and North America. The aim of the present study was to investigate mutations in the algTmucABD operon in mucoid and non-mucoid isolates from Brazilian CF patients. Twenty-seven mucoid and 37 non-mucoid isolates from 40 CF patients chronically infected by P. aeruginosa attending a CF reference center in Brazil were evaluated by sequence analysis. Mutations in mucA were observed in 93{\%} of the mucoid isolates and 54{\%} of the non-mucoid isolates. Among these non-mucoid isolates, 55{\%} were considered revertants, since they also had mutations in algT (algU). Most isolates associated with moderate alginate production presented point mutations in mucB and/or mucD. We identified 30 mutations not previously described in the operon. In conclusion, mutations in mucA were the main mechanism of conversion to mucoidy, and most of the non-mucoid isolates were revertants, but the mechanism of revertance is not fully explained by changes in algT.",
author = "{Candido Ca{\cc}ador}, Nat{\'a}lia and {Paulino da Costa Capizzani}, Carolina and {Gomes Monteiro Marin Torres}, {L{\'i}dia Alice} and Renata Galetti and Oana Ciofu and {da Costa Darini}, {Ana L{\'u}cia} and Niels H{\o}iby",
year = "2018",
doi = "10.1371/journal.pone.0208013",
language = "English",
volume = "13",
pages = "e0208013",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

RIS

TY - JOUR

T1 - Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients

AU - Candido Caçador, Natália

AU - Paulino da Costa Capizzani, Carolina

AU - Gomes Monteiro Marin Torres, Lídia Alice

AU - Galetti, Renata

AU - Ciofu, Oana

AU - da Costa Darini, Ana Lúcia

AU - Høiby, Niels

PY - 2018

Y1 - 2018

N2 - Chronic lung infection by Pseudomonas aeruginosa is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. This is associated with the conversion of the non-mucoid to the mucoid phenotype. However, there is little information about the occurrence of alginate-producing P. aeruginosa in CF patients outside Europe and North America. The aim of the present study was to investigate mutations in the algTmucABD operon in mucoid and non-mucoid isolates from Brazilian CF patients. Twenty-seven mucoid and 37 non-mucoid isolates from 40 CF patients chronically infected by P. aeruginosa attending a CF reference center in Brazil were evaluated by sequence analysis. Mutations in mucA were observed in 93% of the mucoid isolates and 54% of the non-mucoid isolates. Among these non-mucoid isolates, 55% were considered revertants, since they also had mutations in algT (algU). Most isolates associated with moderate alginate production presented point mutations in mucB and/or mucD. We identified 30 mutations not previously described in the operon. In conclusion, mutations in mucA were the main mechanism of conversion to mucoidy, and most of the non-mucoid isolates were revertants, but the mechanism of revertance is not fully explained by changes in algT.

AB - Chronic lung infection by Pseudomonas aeruginosa is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. This is associated with the conversion of the non-mucoid to the mucoid phenotype. However, there is little information about the occurrence of alginate-producing P. aeruginosa in CF patients outside Europe and North America. The aim of the present study was to investigate mutations in the algTmucABD operon in mucoid and non-mucoid isolates from Brazilian CF patients. Twenty-seven mucoid and 37 non-mucoid isolates from 40 CF patients chronically infected by P. aeruginosa attending a CF reference center in Brazil were evaluated by sequence analysis. Mutations in mucA were observed in 93% of the mucoid isolates and 54% of the non-mucoid isolates. Among these non-mucoid isolates, 55% were considered revertants, since they also had mutations in algT (algU). Most isolates associated with moderate alginate production presented point mutations in mucB and/or mucD. We identified 30 mutations not previously described in the operon. In conclusion, mutations in mucA were the main mechanism of conversion to mucoidy, and most of the non-mucoid isolates were revertants, but the mechanism of revertance is not fully explained by changes in algT.

U2 - 10.1371/journal.pone.0208013

DO - 10.1371/journal.pone.0208013

M3 - Journal article

VL - 13

SP - e0208013

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 11

ER -

ID: 56276230