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Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children

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Walker, Melanie R ; Knudsen, Anne S ; Partey, Frederica D ; Bassi, Maria R ; Frank, Asger M ; Castberg, Filip C ; Sarbah, Edem W ; Ofori, Michael F ; Hviid, Lars ; Barfod, Lea. / Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children. I: PLoS One. 2020 ; Bind 15, Nr. 12. s. e0243943.

Bibtex

@article{4846060dd3a34152b4fb1b1ba986ba80,
title = "Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children",
abstract = "Developing a vaccine against Plasmodium falciparum malaria has been challenging, primarily due to high levels of antigen polymorphism and a complex parasite lifecycle. Immunization with the P. falciparum merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 has been shown to give rise to growth inhibitory and synergistic antisera. Therefore, these five merozoite proteins are considered to be promising candidates for a second-generation multivalent malaria vaccine. Nevertheless, little is known about IgG and IgM responses to these antigens in populations that are naturally exposed to P. falciparum. In this study, serum samples from clinically immune adults and malaria exposed children from Ghana were studied to compare levels of IgG and IgM specific for PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5. All five antigens were found to be specifically recognized by both IgM and IgG in serum from clinically immune adults and from children with malaria. Longitudinal analysis of the latter group showed an early, transient IgM response that was followed by IgG, which peaked 14 days after the initial diagnosis. IgG levels and parasitemia did not correlate, whereas parasitemia was weakly positively correlated with IgM levels. These findings show that IgG and IgM specific for merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 are high in children during P. falciparum malaria, but that the IgM induction and decline occurs earlier in infection than that of IgG.",
keywords = "Adolescent, Adult, Aged, Antibodies, Protozoan/immunology, Antigens, Protozoan/immunology, Child, Child, Preschool, Female, Humans, Immunoglobulin G/immunology, Immunoglobulin M/immunology, Infant, Malaria Vaccines/immunology, Malaria, Falciparum/immunology, Male, Merozoites/immunology, Middle Aged, Plasmodium falciparum/immunology, Young Adult",
author = "Walker, {Melanie R} and Knudsen, {Anne S} and Partey, {Frederica D} and Bassi, {Maria R} and Frank, {Asger M} and Castberg, {Filip C} and Sarbah, {Edem W} and Ofori, {Michael F} and Lars Hviid and Lea Barfod",
year = "2020",
doi = "10.1371/journal.pone.0243943",
language = "English",
volume = "15",
pages = "e0243943",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

RIS

TY - JOUR

T1 - Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children

AU - Walker, Melanie R

AU - Knudsen, Anne S

AU - Partey, Frederica D

AU - Bassi, Maria R

AU - Frank, Asger M

AU - Castberg, Filip C

AU - Sarbah, Edem W

AU - Ofori, Michael F

AU - Hviid, Lars

AU - Barfod, Lea

PY - 2020

Y1 - 2020

N2 - Developing a vaccine against Plasmodium falciparum malaria has been challenging, primarily due to high levels of antigen polymorphism and a complex parasite lifecycle. Immunization with the P. falciparum merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 has been shown to give rise to growth inhibitory and synergistic antisera. Therefore, these five merozoite proteins are considered to be promising candidates for a second-generation multivalent malaria vaccine. Nevertheless, little is known about IgG and IgM responses to these antigens in populations that are naturally exposed to P. falciparum. In this study, serum samples from clinically immune adults and malaria exposed children from Ghana were studied to compare levels of IgG and IgM specific for PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5. All five antigens were found to be specifically recognized by both IgM and IgG in serum from clinically immune adults and from children with malaria. Longitudinal analysis of the latter group showed an early, transient IgM response that was followed by IgG, which peaked 14 days after the initial diagnosis. IgG levels and parasitemia did not correlate, whereas parasitemia was weakly positively correlated with IgM levels. These findings show that IgG and IgM specific for merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 are high in children during P. falciparum malaria, but that the IgM induction and decline occurs earlier in infection than that of IgG.

AB - Developing a vaccine against Plasmodium falciparum malaria has been challenging, primarily due to high levels of antigen polymorphism and a complex parasite lifecycle. Immunization with the P. falciparum merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 has been shown to give rise to growth inhibitory and synergistic antisera. Therefore, these five merozoite proteins are considered to be promising candidates for a second-generation multivalent malaria vaccine. Nevertheless, little is known about IgG and IgM responses to these antigens in populations that are naturally exposed to P. falciparum. In this study, serum samples from clinically immune adults and malaria exposed children from Ghana were studied to compare levels of IgG and IgM specific for PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5. All five antigens were found to be specifically recognized by both IgM and IgG in serum from clinically immune adults and from children with malaria. Longitudinal analysis of the latter group showed an early, transient IgM response that was followed by IgG, which peaked 14 days after the initial diagnosis. IgG levels and parasitemia did not correlate, whereas parasitemia was weakly positively correlated with IgM levels. These findings show that IgG and IgM specific for merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 are high in children during P. falciparum malaria, but that the IgM induction and decline occurs earlier in infection than that of IgG.

KW - Adolescent

KW - Adult

KW - Aged

KW - Antibodies, Protozoan/immunology

KW - Antigens, Protozoan/immunology

KW - Child

KW - Child, Preschool

KW - Female

KW - Humans

KW - Immunoglobulin G/immunology

KW - Immunoglobulin M/immunology

KW - Infant

KW - Malaria Vaccines/immunology

KW - Malaria, Falciparum/immunology

KW - Male

KW - Merozoites/immunology

KW - Middle Aged

KW - Plasmodium falciparum/immunology

KW - Young Adult

U2 - 10.1371/journal.pone.0243943

DO - 10.1371/journal.pone.0243943

M3 - Journal article

C2 - 33332459

VL - 15

SP - e0243943

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 12

ER -

ID: 62332776