A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

Yukie M Lloyd; Elise P Ngati; Ali Salanti; Rose G F Leke; Diane W Taylor

doi:10.1038/s41598-017-13900-4

A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

Yukie M Lloyd, Elise P Ngati, Ali Salanti, Rose G F Leke, Diane W Taylor

7 Citationer (Scopus)

Abstract

Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.

Originalsprog	Engelsk
Tidsskrift	Scientific Reports
Vol/bind	7
Udgave nummer	1
Sider (fra-til)	e14705
ISSN	2045-2322
DOI	https://doi.org/10.1038/s41598-017-13900-4
Status	Udgivet - 31 okt. 2017

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10.1038/s41598-017-13900-4

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AU - Taylor, Diane W

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AB - Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.

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A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

Abstract

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