A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici

Peter Skottrup; Stephen Hearty; Hanne Frøkiær; Paul Leonard; Jørn Hejgaard; Richard O'Kennedy; Mogens Nicolaisen; Annemarie Fejer Justesen

A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici

Peter Skottrup, Stephen Hearty, Hanne Frøkiær, Paul Leonard, Jørn Hejgaard, Richard O'Kennedy, Mogens Nicolaisen, Annemarie Fejer Justesen

Klinisk Biokemisk Afdeling

Abstract

This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst

Originalsprog	Udefineret/Ukendt
Publikationsdato	2006
Status	Udgivet - 2006

Citationsformater

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title = "A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici",

abstract = "This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst",

author = "Peter Skottrup and Stephen Hearty and Hanne Fr{\o}ki{\ae}r and Paul Leonard and J{\o}rn Hejgaard and Richard O'Kennedy and Mogens Nicolaisen and Justesen, {Annemarie Fejer}",

year = "2006",

language = "Udefineret/Ukendt",

type = "Other",

}

TY - GEN

T1 - A Surface Plasmon Resonance Immunosensor for Detection of urediniospores from Puccinia striiformis f. sp. tritici

AU - Skottrup, Peter

AU - Hearty, Stephen

AU - Frøkiær, Hanne

AU - Leonard, Paul

AU - Hejgaard, Jørn

AU - O'Kennedy, Richard

AU - Nicolaisen, Mogens

AU - Justesen, Annemarie Fejer

PY - 2006

Y1 - 2006

N2 - This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst

AB - This study describes a generic biosensing principle for detection of fungal spores using surface plasmon resonance (SPR). The approach involves the use of a mouse monoclonal antibody (mAb) and a SPR sensor for label-free detection of the model organism Puccinia striiformis f.sp. tritici (Pst). We developed mAbs towards intact whole spores and used a subtractive inhibition format for detection of spores in solution. Assay conditions was optimised and a detection limit of 3.9 x 103 spores/ml was achieved. We further examined spiked Pst samples in a background of a related spore and found that Pst quantification was possible in this complex matrix. To the best of our knowledge, this study simultaneously represents the first use of SPR technology for fungal spore detection and the first report of a successful biosensor-based detection strategy for Pst

M3 - Andet bidrag

ER -