A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection

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Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Harvard

Zanghì, G, Vembar, SS, Baumgarten, S, Ding, S, Guizetti, J, Bryant, JM, Mattei, D, Jensen, ATR, Rénia, L, Goh, YS, Sauerwein, R, Hermsen, CC, Franetich, J-F, Bordessoulles, M, Silvie, O, Soulard, V, Scatton, O, Chen, P, Mecheri, S, Mazier, D & Scherf, A 2018, 'A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection' Cell Reports, bind 22, nr. 11, s. 2951-2963. https://doi.org/10.1016/j.celrep.2018.02.075

APA

Zanghì, G., Vembar, S. S., Baumgarten, S., Ding, S., Guizetti, J., Bryant, J. M., ... Scherf, A. (2018). A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection. Cell Reports, 22(11), 2951-2963. https://doi.org/10.1016/j.celrep.2018.02.075

CBE

Zanghì G, Vembar SS, Baumgarten S, Ding S, Guizetti J, Bryant JM, Mattei D, Jensen ATR, Rénia L, Goh YS, Sauerwein R, Hermsen CC, Franetich J-F, Bordessoulles M, Silvie O, Soulard V, Scatton O, Chen P, Mecheri S, Mazier D, Scherf A. 2018. A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection. Cell Reports. 22(11):2951-2963. https://doi.org/10.1016/j.celrep.2018.02.075

MLA

Zanghì, Gigliola o.a.. "A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection". Cell Reports. 2018, 22(11). 2951-2963. https://doi.org/10.1016/j.celrep.2018.02.075

Vancouver

Zanghì G, Vembar SS, Baumgarten S, Ding S, Guizetti J, Bryant JM o.a. A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection. Cell Reports. 2018 mar 13;22(11):2951-2963. https://doi.org/10.1016/j.celrep.2018.02.075

Author

Zanghì, Gigliola ; Vembar, Shruthi S ; Baumgarten, Sebastian ; Ding, Shuai ; Guizetti, Julien ; Bryant, Jessica M ; Mattei, Denise ; Jensen, Anja T R ; Rénia, Laurent ; Goh, Yun Shan ; Sauerwein, Robert ; Hermsen, Cornelus C ; Franetich, Jean-François ; Bordessoulles, Mallaury ; Silvie, Olivier ; Soulard, Valérie ; Scatton, Olivier ; Chen, Patty ; Mecheri, Salah ; Mazier, Dominique ; Scherf, Artur. / A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection. I: Cell Reports. 2018 ; Bind 22, Nr. 11. s. 2951-2963.

Bibtex

@article{a94fb776e89d47c69b72ec9154618cf4,

title = "A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection",

abstract = "Heterochromatin plays a central role in the process of immune evasion, pathogenesis, and transmission of the malaria parasite Plasmodium falciparum during blood stage infection. Here, we use ChIP sequencing to demonstrate that sporozoites from mosquito salivary glands expand heterochromatin at subtelomeric regions to silence blood-stage-specific genes. Our data also revealed that heterochromatin enrichment is predictive of the transcription status of clonally variant genes members that mediate cytoadhesion in blood stage parasites. A specific member (here called NF54varsporo) of the var gene family remains euchromatic, and the resultant PfEMP1 (NF54_SpzPfEMP1) is expressed at the sporozoite surface. NF54_SpzPfEMP1-specific antibodies efficiently block hepatocyte infection in a strain-specific manner. Furthermore, human volunteers immunized with infective sporozoites developed antibodies against NF54_SpzPfEMP1. Overall, we show that the epigenetic signature of var genes is reset in mosquito stages. Moreover, the identification of a strain-specific sporozoite PfEMP1 is highly relevant for vaccine design based on sporozoites.",

author = "Gigliola Zangh{\`i} and Vembar, {Shruthi S} and Sebastian Baumgarten and Shuai Ding and Julien Guizetti and Bryant, {Jessica M} and Denise Mattei and Jensen, {Anja T R} and Laurent R{\'e}nia and Goh, {Yun Shan} and Robert Sauerwein and Hermsen, {Cornelus C} and Jean-Fran{\cc}ois Franetich and Mallaury Bordessoulles and Olivier Silvie and Val{\'e}rie Soulard and Olivier Scatton and Patty Chen and Salah Mecheri and Dominique Mazier and Artur Scherf",

year = "2018",

month = "3",

day = "13",

doi = "10.1016/j.celrep.2018.02.075",

language = "English",

volume = "22",

pages = "2951--2963",

journal = "Cell Reports",

publisher = "Cell Press",

number = "11",

}

RIS

TY - JOUR

T1 - A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection

AU - Zanghì, Gigliola

AU - Vembar, Shruthi S

AU - Baumgarten, Sebastian

AU - Ding, Shuai

AU - Guizetti, Julien

AU - Bryant, Jessica M

AU - Mattei, Denise

AU - Jensen, Anja T R

AU - Rénia, Laurent

AU - Goh, Yun Shan

AU - Sauerwein, Robert

AU - Hermsen, Cornelus C

AU - Franetich, Jean-François

AU - Bordessoulles, Mallaury

AU - Silvie, Olivier

AU - Soulard, Valérie

AU - Scatton, Olivier

AU - Chen, Patty

AU - Mecheri, Salah

AU - Mazier, Dominique

AU - Scherf, Artur

PY - 2018/3/13

Y1 - 2018/3/13

N2 - Heterochromatin plays a central role in the process of immune evasion, pathogenesis, and transmission of the malaria parasite Plasmodium falciparum during blood stage infection. Here, we use ChIP sequencing to demonstrate that sporozoites from mosquito salivary glands expand heterochromatin at subtelomeric regions to silence blood-stage-specific genes. Our data also revealed that heterochromatin enrichment is predictive of the transcription status of clonally variant genes members that mediate cytoadhesion in blood stage parasites. A specific member (here called NF54varsporo) of the var gene family remains euchromatic, and the resultant PfEMP1 (NF54_SpzPfEMP1) is expressed at the sporozoite surface. NF54_SpzPfEMP1-specific antibodies efficiently block hepatocyte infection in a strain-specific manner. Furthermore, human volunteers immunized with infective sporozoites developed antibodies against NF54_SpzPfEMP1. Overall, we show that the epigenetic signature of var genes is reset in mosquito stages. Moreover, the identification of a strain-specific sporozoite PfEMP1 is highly relevant for vaccine design based on sporozoites.

AB - Heterochromatin plays a central role in the process of immune evasion, pathogenesis, and transmission of the malaria parasite Plasmodium falciparum during blood stage infection. Here, we use ChIP sequencing to demonstrate that sporozoites from mosquito salivary glands expand heterochromatin at subtelomeric regions to silence blood-stage-specific genes. Our data also revealed that heterochromatin enrichment is predictive of the transcription status of clonally variant genes members that mediate cytoadhesion in blood stage parasites. A specific member (here called NF54varsporo) of the var gene family remains euchromatic, and the resultant PfEMP1 (NF54_SpzPfEMP1) is expressed at the sporozoite surface. NF54_SpzPfEMP1-specific antibodies efficiently block hepatocyte infection in a strain-specific manner. Furthermore, human volunteers immunized with infective sporozoites developed antibodies against NF54_SpzPfEMP1. Overall, we show that the epigenetic signature of var genes is reset in mosquito stages. Moreover, the identification of a strain-specific sporozoite PfEMP1 is highly relevant for vaccine design based on sporozoites.

U2 - 10.1016/j.celrep.2018.02.075

DO - 10.1016/j.celrep.2018.02.075

M3 - Journal article

VL - 22

SP - 2951

EP - 2963

JO - Cell Reports

JF - Cell Reports

IS - 11

ER -

ID: 56467130