Harvard
Zanghì, G, Vembar, SS, Baumgarten, S, Ding, S, Guizetti, J, Bryant, JM, Mattei, D
, Jensen, ATR, Rénia, L, Goh, YS, Sauerwein, R, Hermsen, CC, Franetich, J-F, Bordessoulles, M, Silvie, O, Soulard, V, Scatton, O, Chen, P, Mecheri, S, Mazier, D & Scherf, A 2018, '
A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection'
Cell Reports, bind 22, nr. 11, s. 2951-2963.
https://doi.org/10.1016/j.celrep.2018.02.075
APA
Zanghì, G., Vembar, S. S., Baumgarten, S., Ding, S., Guizetti, J., Bryant, J. M., ... Scherf, A. (2018).
A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
Cell Reports,
22(11), 2951-2963.
https://doi.org/10.1016/j.celrep.2018.02.075
CBE
Zanghì G, Vembar SS, Baumgarten S, Ding S, Guizetti J, Bryant JM, Mattei D
, Jensen ATR, Rénia L, Goh YS, Sauerwein R, Hermsen CC, Franetich J-F, Bordessoulles M, Silvie O, Soulard V, Scatton O, Chen P, Mecheri S, Mazier D, Scherf A. 2018.
A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
Cell Reports. 22(11):2951-2963.
https://doi.org/10.1016/j.celrep.2018.02.075
MLA
Vancouver
Author
Zanghì, Gigliola ; Vembar, Shruthi S ; Baumgarten, Sebastian ; Ding, Shuai ; Guizetti, Julien ; Bryant, Jessica M ; Mattei, Denise
; Jensen, Anja T R ; Rénia, Laurent ; Goh, Yun Shan ; Sauerwein, Robert ; Hermsen, Cornelus C ; Franetich, Jean-François ; Bordessoulles, Mallaury ; Silvie, Olivier ; Soulard, Valérie ; Scatton, Olivier ; Chen, Patty ; Mecheri, Salah ; Mazier, Dominique ; Scherf, Artur. /
A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection. I:
Cell Reports. 2018 ; Bind 22, Nr. 11. s. 2951-2963.
Bibtex
@article{a94fb776e89d47c69b72ec9154618cf4,
title = "A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection",
abstract = "Heterochromatin plays a central role in the process of immune evasion, pathogenesis, and transmission of the malaria parasite Plasmodium falciparum during blood stage infection. Here, we use ChIP sequencing to demonstrate that sporozoites from mosquito salivary glands expand heterochromatin at subtelomeric regions to silence blood-stage-specific genes. Our data also revealed that heterochromatin enrichment is predictive of the transcription status of clonally variant genes members that mediate cytoadhesion in blood stage parasites. A specific member (here called NF54varsporo) of the var gene family remains euchromatic, and the resultant PfEMP1 (NF54_SpzPfEMP1) is expressed at the sporozoite surface. NF54_SpzPfEMP1-specific antibodies efficiently block hepatocyte infection in a strain-specific manner. Furthermore, human volunteers immunized with infective sporozoites developed antibodies against NF54_SpzPfEMP1. Overall, we show that the epigenetic signature of var genes is reset in mosquito stages. Moreover, the identification of a strain-specific sporozoite PfEMP1 is highly relevant for vaccine design based on sporozoites.",
author = "Gigliola Zangh{\`i} and Vembar, {Shruthi S} and Sebastian Baumgarten and Shuai Ding and Julien Guizetti and Bryant, {Jessica M} and Denise Mattei and Jensen, {Anja T R} and Laurent R{\'e}nia and Goh, {Yun Shan} and Robert Sauerwein and Hermsen, {Cornelus C} and Jean-Fran{\cc}ois Franetich and Mallaury Bordessoulles and Olivier Silvie and Val{\'e}rie Soulard and Olivier Scatton and Patty Chen and Salah Mecheri and Dominique Mazier and Artur Scherf",
note = "Copyright {\circledC} 2018 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2018",
month = "3",
day = "13",
doi = "10.1016/j.celrep.2018.02.075",
language = "English",
volume = "22",
pages = "2951--2963",
journal = "Cell Reports",
publisher = "Cell Press",
number = "11",
}
RIS
TY - JOUR
T1 - A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection
AU - Zanghì, Gigliola
AU - Vembar, Shruthi S
AU - Baumgarten, Sebastian
AU - Ding, Shuai
AU - Guizetti, Julien
AU - Bryant, Jessica M
AU - Mattei, Denise
AU - Jensen, Anja T R
AU - Rénia, Laurent
AU - Goh, Yun Shan
AU - Sauerwein, Robert
AU - Hermsen, Cornelus C
AU - Franetich, Jean-François
AU - Bordessoulles, Mallaury
AU - Silvie, Olivier
AU - Soulard, Valérie
AU - Scatton, Olivier
AU - Chen, Patty
AU - Mecheri, Salah
AU - Mazier, Dominique
AU - Scherf, Artur
N1 - Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2018/3/13
Y1 - 2018/3/13
N2 - Heterochromatin plays a central role in the process of immune evasion, pathogenesis, and transmission of the malaria parasite Plasmodium falciparum during blood stage infection. Here, we use ChIP sequencing to demonstrate that sporozoites from mosquito salivary glands expand heterochromatin at subtelomeric regions to silence blood-stage-specific genes. Our data also revealed that heterochromatin enrichment is predictive of the transcription status of clonally variant genes members that mediate cytoadhesion in blood stage parasites. A specific member (here called NF54varsporo) of the var gene family remains euchromatic, and the resultant PfEMP1 (NF54_SpzPfEMP1) is expressed at the sporozoite surface. NF54_SpzPfEMP1-specific antibodies efficiently block hepatocyte infection in a strain-specific manner. Furthermore, human volunteers immunized with infective sporozoites developed antibodies against NF54_SpzPfEMP1. Overall, we show that the epigenetic signature of var genes is reset in mosquito stages. Moreover, the identification of a strain-specific sporozoite PfEMP1 is highly relevant for vaccine design based on sporozoites.
AB - Heterochromatin plays a central role in the process of immune evasion, pathogenesis, and transmission of the malaria parasite Plasmodium falciparum during blood stage infection. Here, we use ChIP sequencing to demonstrate that sporozoites from mosquito salivary glands expand heterochromatin at subtelomeric regions to silence blood-stage-specific genes. Our data also revealed that heterochromatin enrichment is predictive of the transcription status of clonally variant genes members that mediate cytoadhesion in blood stage parasites. A specific member (here called NF54varsporo) of the var gene family remains euchromatic, and the resultant PfEMP1 (NF54_SpzPfEMP1) is expressed at the sporozoite surface. NF54_SpzPfEMP1-specific antibodies efficiently block hepatocyte infection in a strain-specific manner. Furthermore, human volunteers immunized with infective sporozoites developed antibodies against NF54_SpzPfEMP1. Overall, we show that the epigenetic signature of var genes is reset in mosquito stages. Moreover, the identification of a strain-specific sporozoite PfEMP1 is highly relevant for vaccine design based on sporozoites.
U2 - 10.1016/j.celrep.2018.02.075
DO - 10.1016/j.celrep.2018.02.075
M3 - Journal article
VL - 22
SP - 2951
EP - 2963
JO - Cell Reports
JF - Cell Reports
IS - 11
ER -