Forskning
Udskriv Udskriv
Switch language
Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

A Single Dose of Psilocybin Increases Synaptic Density and Decreases 5-HT2A Receptor Density in the Pig Brain

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Effects of a single dose of psilocybin on behaviour, brain 5-HT2A receptor occupancy and gene expression in the pig

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Plasma psilocin critically determines behavioral and neurobiological effects of psilocybin

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Imaging of the Serotonin System: Radiotracers and Applications in Memory Disorders

    Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiUndervisning

  4. Metaboliske, endokrine og andre systemiske sygdomme

    Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiUndervisning

Vis graf over relationer

A single dose of psilocybin, a psychedelic and serotonin 2A receptor (5-HT2AR) agonist, may be associated with antidepressant effects. The mechanism behind its antidepressive action is unknown but could be linked to increased synaptogenesis and down-regulation of cerebral 5-HT2AR. Here, we investigate if a single psychedelic dose of psilocybin changes synaptic vesicle protein 2A (SV2A) and 5-HT2AR density in the pig brain. Twenty-four awake pigs received either 0.08 mg/kg psilocybin or saline intravenously. Twelve pigs (n = 6/intervention) were euthanized one day post-injection, while the remaining twelve pigs were euthanized seven days post-injection (n = 6/intervention). We performed autoradiography on hippocampus and prefrontal cortex (PFC) sections with [3H]UCB-J (SV2A), [3H]MDL100907 (5-HT2AR antagonist) and [3H]Cimbi-36 (5-HT2AR agonist). One day post psilocybin injection, we observed 4.42% higher hippocampal SV2A density and lowered hippocampal and PFC 5-HT2AR density (-15.21% to -50.19%). These differences were statistically significant in the hippocampus for all radioligands and in the PFC for [3H]Cimbi-36 only. Seven days post-intervention, there was still significantly higher SV2A density in the hippocampus (+9.24%) and the PFC (+6.10%), whereas there were no longer any differences in 5-HT2AR density. Our findings suggest that psilocybin causes increased persistent synaptogenesis and an acute decrease in 5-HT2AR density, which may play a role in psilocybin's antidepressive effects.

OriginalsprogEngelsk
Artikelnummer835
TidsskriftInternational Journal of Molecular Sciences
Vol/bind22
Udgave nummer2
Sider (fra-til)1-14
Antal sider14
ISSN1661-6596
DOI
StatusUdgivet - 15 jan. 2021

ID: 62238907