A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes

Irina Kratchmarova, Dario E Kalume, Blagoy Blagoev, Philipp E Scherer, Alexandre V Podtelejnikov, Henrik Molina, Perry E Bickel, Jens S Andersen, Minerva M Fernandez, Jacob Bunkenborg, Peter Roepstorff, Karsten Kristiansen, Harvey F Lodish, Matthias Mann, Akhilesh Pandey

235 Citationer (Scopus)


We have undertaken a systematic proteomic approach to purify and identify secreted factors that are differentially expressed in preadipocytes versus adipocytes. Using one-dimensional gel electrophoresis combined with nanoelectrospray tandem mass spectrometry, proteins that were specifically secreted by 3T3-L1 preadipocytes or adipocytes were identified. In addition to a number of previously reported molecules that are up- or down-regulated during this differentiation process (adipsin, adipocyte complement-related protein 30 kDa, complement C3, and fibronectin), we identified four secreted molecules that have not been shown previously to be expressed differentially during the process of adipogenesis. Pigment epithelium-derived factor, a soluble molecule with potent antiangiogenic properties, was found to be highly secreted by preadipocytes but not adipocytes. Conversely, we found hippocampal cholinergic neurostimulating peptide, neutrophil gelatinase-associated lipocalin, and haptoglobin to be expressed highly by mature adipocytes. We also used liquid chromatography-based separation followed by automated tandem mass spectrometry to identify proteins secreted by mature adipocytes. Several additional secreted proteins including resistin, secreted acidic cysteine-rich glycoprotein/osteonectin, stromal cell-derived factor-1, cystatin C, gelsolin, and matrix metalloprotease-2 were identified by this method. To our knowledge, this is the first study to identify several novel secreted proteins by adipocytes by a proteomic approach using mass spectrometry.
TidsskriftMolecular & cellular proteomics : MCP
Udgave nummer3
Sider (fra-til)213-22
Antal sider10
StatusUdgivet - 2002
Udgivet eksterntJa


Dyk ned i forskningsemnerne om 'A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes'. Sammen danner de et unikt fingeraftryk.