A platform for efficient establishment and drug-response profiling of high-grade serous ovarian cancer organoids

Wojciech Senkowski; Laura Gall-Mas; Matías Marín Falco; Yilin Li; Kari Lavikka; Mette C Kriegbaum; Jaana Oikkonen; Daria Bulanova; Elin J Pietras; Karolin Voßgröne; Yan-Jun Chen; Erdogan Pekcan Erkan; Jun Dai; Anastasia Lundgren; Mia Kristine Grønning Høg; Ida Marie Larsen; Tarja Lamminen; Katja Kaipio; Jutta Huvila; Anni Virtanen; Lars Engelholm; Pernille Christiansen; Eric Santoni-Rugiu; Kaisa Huhtinen; Olli Carpén; Johanna Hynninen; Sampsa Hautaniemi; Anna Vähärautio; Krister Wennerberg

doi:10.1016/j.devcel.2023.04.012

A platform for efficient establishment and drug-response profiling of high-grade serous ovarian cancer organoids

Wojciech Senkowski^*, Laura Gall-Mas, Matías Marín Falco, Yilin Li, Kari Lavikka, Mette C Kriegbaum, Jaana Oikkonen, Daria Bulanova, Elin J Pietras, Karolin Voßgröne, Yan-Jun Chen, Erdogan Pekcan Erkan, Jun Dai, Anastasia Lundgren, Mia Kristine Grønning Høg, Ida Marie Larsen, Tarja Lamminen, Katja Kaipio, Jutta Huvila, Anni VirtanenLars Engelholm, Pernille Christiansen, Eric Santoni-Rugiu, Kaisa Huhtinen, Olli Carpén, Johanna Hynninen, Sampsa Hautaniemi, Anna Vähärautio, Krister Wennerberg^*

^*Corresponding author af dette arbejde

29 Citationer (Scopus)

Abstract

The broad research use of organoids from high-grade serous ovarian cancer (HGSC) has been hampered by low culture success rates and limited availability of fresh tumor material. Here, we describe a method for generation and long-term expansion of HGSC organoids with efficacy markedly improved over previous reports (53% vs. 23%-38%). We established organoids from cryopreserved material, demonstrating the feasibility of using viably biobanked tissue for HGSC organoid derivation. Genomic, histologic, and single-cell transcriptomic analyses revealed that organoids recapitulated genetic and phenotypic features of original tumors. Organoid drug responses correlated with clinical treatment outcomes, although in a culture conditions-dependent manner and only in organoids maintained in human plasma-like medium (HPLM). Organoids from consenting patients are available to the research community through a public biobank and organoid genomic data are explorable through an interactive online tool. Taken together, this resource facilitates the application of HGSC organoids in basic and translational ovarian cancer research.

Originalsprog	Engelsk
Tidsskrift	Developmental Cell
Vol/bind	58
Udgave nummer	12
Sider (fra-til)	1106-1121.e7
ISSN	1534-5807
DOI	https://doi.org/10.1016/j.devcel.2023.04.012
Status	Udgivet - 19 jun. 2023

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10.1016/j.devcel.2023.04.012

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Senkowski, W., Gall-Mas, L., Falco, M. M., Li, Y., Lavikka, K., Kriegbaum, M. C., Oikkonen, J., Bulanova, D., Pietras, E. J., Voßgröne, K., Chen, Y.-J., Erkan, E. P., Dai, J., Lundgren, A., Grønning Høg, M. K., Larsen, I. M., Lamminen, T., Kaipio, K., Huvila, J., ... Wennerberg, K. (2023). A platform for efficient establishment and drug-response profiling of high-grade serous ovarian cancer organoids. Developmental Cell, 58(12), 1106-1121.e7. https://doi.org/10.1016/j.devcel.2023.04.012

Senkowski, W, Gall-Mas, L, Falco, MM, Li, Y, Lavikka, K, Kriegbaum, MC, Oikkonen, J, Bulanova, D, Pietras, EJ, Voßgröne, K, Chen, Y-J, Erkan, EP, Dai, J, Lundgren, A, Grønning Høg, MK , Larsen, IM, Lamminen, T, Kaipio, K, Huvila, J, Virtanen, A, Engelholm, L, Christiansen, P, Santoni-Rugiu, E, Huhtinen, K, Carpén, O, Hynninen, J, Hautaniemi, S, Vähärautio, A & Wennerberg, K 2023, 'A platform for efficient establishment and drug-response profiling of high-grade serous ovarian cancer organoids', Developmental Cell, bind 58, nr. 12, s. 1106-1121.e7. https://doi.org/10.1016/j.devcel.2023.04.012

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abstract = "The broad research use of organoids from high-grade serous ovarian cancer (HGSC) has been hampered by low culture success rates and limited availability of fresh tumor material. Here, we describe a method for generation and long-term expansion of HGSC organoids with efficacy markedly improved over previous reports (53% vs. 23%-38%). We established organoids from cryopreserved material, demonstrating the feasibility of using viably biobanked tissue for HGSC organoid derivation. Genomic, histologic, and single-cell transcriptomic analyses revealed that organoids recapitulated genetic and phenotypic features of original tumors. Organoid drug responses correlated with clinical treatment outcomes, although in a culture conditions-dependent manner and only in organoids maintained in human plasma-like medium (HPLM). Organoids from consenting patients are available to the research community through a public biobank and organoid genomic data are explorable through an interactive online tool. Taken together, this resource facilitates the application of HGSC organoids in basic and translational ovarian cancer research.",

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AU - Senkowski, Wojciech

AU - Gall-Mas, Laura

AU - Falco, Matías Marín

AU - Li, Yilin

AU - Lavikka, Kari

AU - Kriegbaum, Mette C

AU - Oikkonen, Jaana

AU - Bulanova, Daria

AU - Pietras, Elin J

AU - Voßgröne, Karolin

AU - Chen, Yan-Jun

AU - Erkan, Erdogan Pekcan

AU - Dai, Jun

AU - Lundgren, Anastasia

AU - Grønning Høg, Mia Kristine

AU - Larsen, Ida Marie

AU - Lamminen, Tarja

AU - Kaipio, Katja

AU - Huvila, Jutta

AU - Virtanen, Anni

AU - Engelholm, Lars

AU - Christiansen, Pernille

AU - Santoni-Rugiu, Eric

AU - Huhtinen, Kaisa

AU - Carpén, Olli

AU - Hynninen, Johanna

AU - Hautaniemi, Sampsa

AU - Vähärautio, Anna

AU - Wennerberg, Krister

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N2 - The broad research use of organoids from high-grade serous ovarian cancer (HGSC) has been hampered by low culture success rates and limited availability of fresh tumor material. Here, we describe a method for generation and long-term expansion of HGSC organoids with efficacy markedly improved over previous reports (53% vs. 23%-38%). We established organoids from cryopreserved material, demonstrating the feasibility of using viably biobanked tissue for HGSC organoid derivation. Genomic, histologic, and single-cell transcriptomic analyses revealed that organoids recapitulated genetic and phenotypic features of original tumors. Organoid drug responses correlated with clinical treatment outcomes, although in a culture conditions-dependent manner and only in organoids maintained in human plasma-like medium (HPLM). Organoids from consenting patients are available to the research community through a public biobank and organoid genomic data are explorable through an interactive online tool. Taken together, this resource facilitates the application of HGSC organoids in basic and translational ovarian cancer research.

AB - The broad research use of organoids from high-grade serous ovarian cancer (HGSC) has been hampered by low culture success rates and limited availability of fresh tumor material. Here, we describe a method for generation and long-term expansion of HGSC organoids with efficacy markedly improved over previous reports (53% vs. 23%-38%). We established organoids from cryopreserved material, demonstrating the feasibility of using viably biobanked tissue for HGSC organoid derivation. Genomic, histologic, and single-cell transcriptomic analyses revealed that organoids recapitulated genetic and phenotypic features of original tumors. Organoid drug responses correlated with clinical treatment outcomes, although in a culture conditions-dependent manner and only in organoids maintained in human plasma-like medium (HPLM). Organoids from consenting patients are available to the research community through a public biobank and organoid genomic data are explorable through an interactive online tool. Taken together, this resource facilitates the application of HGSC organoids in basic and translational ovarian cancer research.

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KW - Genomics

KW - Humans

KW - Organoids/pathology

KW - Ovarian Neoplasms/drug therapy

KW - 3D cell culture, culture conditions

KW - high-grade serous ovarian cancer

KW - organoids

KW - HPLM

KW - personalized medicine

KW - functional precision medicine

KW - scRNA-seq

KW - organoid biobank

KW - tumor models

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U2 - 10.1016/j.devcel.2023.04.012

DO - 10.1016/j.devcel.2023.04.012

M3 - Journal article

C2 - 37148882

SN - 1534-5807

VL - 58

SP - 1106-1121.e7

JO - Developmental Cell

JF - Developmental Cell

IS - 12

ER -

A platform for efficient establishment and drug-response profiling of high-grade serous ovarian cancer organoids

Abstract

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