A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

D V Agoston; J Fahrenkrug; J D Mikkelsen; V P Whittaker

doi:10.1016/0196-9781(89)90144-7

A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

D V Agoston, J Fahrenkrug, J D Mikkelsen, V P Whittaker

8 Citationer (Scopus)

Abstract

When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.

Originalsprog	Engelsk
Tidsskrift	Peptides
Vol/bind	10
Udgave nummer	3
Sider (fra-til)	571-3
Antal sider	3
ISSN	0196-9781
DOI	https://doi.org/10.1016/0196-9781(89)90144-7
Status	Udgivet - 1989
Udgivet eksternt	Ja

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10.1016/0196-9781(89)90144-7

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@article{ca2f7e228b2a45949784b1bb51d5be86,

title = "A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum",

abstract = "When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.",

keywords = "Animals, Centrifugation, Zonal, Chromatography, Gel, Guinea Pigs, Ileum/innervation, Myenteric Plexus/analysis, Neurons/analysis, Peptide PHI/analysis, Radioimmunoassay, Vasoactive Intestinal Peptide/analysis",

author = "Agoston, {D V} and J Fahrenkrug and Mikkelsen, {J D} and Whittaker, {V P}",

year = "1989",

doi = "10.1016/0196-9781(89)90144-7",

language = "English",

volume = "10",

pages = "571--3",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc",

number = "3",

}

TY - JOUR

T1 - A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

AU - Agoston, D V

AU - Fahrenkrug, J

AU - Mikkelsen, J D

AU - Whittaker, V P

PY - 1989

Y1 - 1989

N2 - When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.

AB - When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.

KW - Animals

KW - Centrifugation, Zonal

KW - Chromatography, Gel

KW - Guinea Pigs

KW - Ileum/innervation

KW - Myenteric Plexus/analysis

KW - Neurons/analysis

KW - Peptide PHI/analysis

KW - Radioimmunoassay

KW - Vasoactive Intestinal Peptide/analysis

U2 - 10.1016/0196-9781(89)90144-7

DO - 10.1016/0196-9781(89)90144-7

M3 - Journal article

C2 - 2780416

SN - 0196-9781

VL - 10

SP - 571

EP - 573

JO - Peptides

JF - Peptides

IS - 3

ER -

A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

Abstract

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