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Region Hovedstaden - en del af Københavns Universitetshospital
Udgivet

A novel xenograft model of cutaneous T-cell lymphoma

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Clinicopathological Features of Ocular Adnexal Mantle-Cell Lymphoma in an International Multicenter Cohort

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Global hypomethylation is an independent prognostic factor in diffuse large B cell lymphoma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Lymphoma of the Eyelid - An International Multicenter Retrospective Study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Thorbjørn Frej Krejsgaard
  • Katharina Luise Maria Kopp
  • Elisabeth Ralfkiaer
  • Ayelah E Willumsgaard
  • Karsten Wessel Kam Eriksen
  • Tord Evert Sigfried Labuda
  • Susanne Rasmussen
  • Anne-Merete Mathiesen
  • Carsten Geisler
  • Britt Thyssing Lauenborg
  • Jürgen C Becker
  • Qian Zhang
  • Mariusz A Wasik
  • Niels Odum
  • Anders Woetmann Andersen
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Cutaneous T-cell lymphomas (CTCLs) are characterized by accumulation of malignant T cells in the skin. Early disease resembles benign skin disorders but during disease progression cutaneous tumors develop, and eventually the malignant T cells can spread to lymph nodes and internal organs. However, because of the lack of suitable animal models, little is known about the mechanisms driving CTCL development and progression in vivo. Here, we describe a novel xenograft model of tumor stage CTCL, where malignant T cells (MyLa2059) are transplanted to NOD/SCID-B2m(-/-) (NOD.Cg-Prkdc(scid) B2m(tm1Unc) /J) mice. Subcutaneous transplantation of the malignant T cells led to rapid tumor formation in 43 of 48 transplantations, whereas transplantation of non-malignant T cells isolated from the same donor did not result in tumor development. Importantly, the tumor growth was significantly suppressed in mice treated with vorinostat when compared to mice treated with vehicle. Furthermore, in most mice the tumors displayed subcutaneous and/or lymphatic dissemination. Histological, immunohistochemical and flow cytometric analyses confirmed that both tumors at the inoculation site, as well as distant subcutaneous and lymphatic tumors, originated from the transplanted malignant T cells. In conclusion, we describe a novel mouse model of tumor stage CTCL for future studies of disease dissemination and preclinical evaluations of new therapeutic strategies.
OriginalsprogEngelsk
TidsskriftExperimental Dermatology
Vol/bind19
Udgave nummer12
Sider (fra-til)1096-102
Antal sider7
ISSN0906-6705
DOI
StatusUdgivet - 1 dec. 2010

ID: 31056750