A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking

N Behrendt, E Rønne, K Danø

    22 Citationer (Scopus)

    Abstract

    Radiolabeled pro-urokinase plasminogen activator (pro-uPA) was cross-linked to a specific protein on the surface of human monocyte-like U937 cells in a reaction catalyzed by tissue transglutaminase. The conjugate formed with this unknown component had a much higher molecular weight (apparent M(r) 250,000-300,000) than the complex of pro-uPA and the urokinase plasminogen activator receptor (uPAR). There was a strong preference for the pro-form of uPA. The conjugate was recognized by antibodies against uPA but not by anti-uPAR antibodies. Nevertheless, the blocking of uPAR with a monoclonal antibody abolished the formation of the conjugate, thus showing a role of uPAR in this process.

    OriginalsprogEngelsk
    TidsskriftF E B S Letters
    Vol/bind336
    Udgave nummer3
    Sider (fra-til)394-6
    Antal sider3
    ISSN0014-5793
    StatusUdgivet - 28 dec. 1993

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