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Region Hovedstaden - en del af Københavns Universitetshospital
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A novel -192c/g mutation in the proximal P2 promoter of the hepatocyte nuclear factor-4 alpha gene (HNF4A) associates with late-onset diabetes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

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  • Jakob Ek
  • Sara Hansen
  • Maria Stenkil Lajer
  • Carine Nicot
  • Trine W Boesgaard
  • Stepanka Pruhova
  • Anders Johansen
  • Anders Albrechtsen
  • Knud Yderstraede
  • Jeannet Lauenborg
  • Marcelina Parrizas
  • Sylvia F Boj
  • Torben Jørgensen
  • Knut Borch-Johnsen
  • Peter Damm
  • Jorge Ferrer
  • Jan Lebl
  • Oluf Pedersen
  • Torben Hansen
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Recently, it has been shown that mutations in the P2 promoter of the hepatocyte nuclear factor (HNF)-4 alpha gene (HNF4A) cause maturity-onset diabetes of the young (MODY), while single nucleotide polymorphisms in this locus are associated with type 2 diabetes. In this study, we examined 1,189 bp of the P2 promoter and the associated exon 1D of HNF4A for variations associated with diabetes in 114 patients with type 2 diabetes, 72 MODYX probands, and 85 women with previous gestational diabetes mellitus. A -192c/g mutation was found in five patients. We screened 1,587 diabetic subjects and 4,812 glucose-tolerant subjects for the -192c/g mutation and identified 5 diabetic and 1 glucose-tolerant mutation carriers (P=0.004). Examination of the families showed that carriers of the -192c/g mutation had a significantly impaired glucose-stimulated insulin release and lower levels of serum total cholesterol compared with matched control subjects. Furthermore, the mutation disrupted the binding of an unidentified sequence-specific DNA binding complex present in human islet extracts. Also, two novel linked polymorphisms in the P2 promoter at positions -1107g/t and -858c/t were identified. These variants were not significantly associated with type 2 diabetes or any pre-diabetic traits. In conclusion, a rare, novel mutation that disrupts a protein binding site in the pancreatic HNF4A promoter associates with late-onset diabetes.
OriginalsprogEngelsk
TidsskriftDiabetes
Vol/bind55
Udgave nummer6
Sider (fra-til)1869-73
Antal sider5
ISSN0012-1797
DOI
StatusUdgivet - 1 jun. 2006

ID: 31043553