TY - JOUR
T1 - A monoclonal antibody as a marker for carcinoma in situ germ cells of the human adult testis
AU - Giwercman, A
AU - Marks, A
AU - Bailey, D
AU - Baumal, R
AU - Skakkebaek, N E
PY - 1988/8
Y1 - 1988/8
N2 - Carcinoma in situ of the testis (CIS) is a precursor of invasive testicular germ cell tumours. The diagnosis of CIS is however often missed when conventional histological techniques are used. No specific immunological marker for CIS germ cells of the testis has been demonstrated previously. A novel monoclonal antibody, M2A, reacting with malignant germ cells of seminomas has recently been developed. Using the immunoperoxidase reaction on tissue sections, we tested the reactivity of M2A with CIS germ cells of the human adult testis. Positive reaction was found in 19 of 20 testicular specimens showing CIS, whereas no staining was found in 39 testicular biopsies without CIS. Thus, M2A may serve as a diagnostic marker in detection of CIS germ cells.
AB - Carcinoma in situ of the testis (CIS) is a precursor of invasive testicular germ cell tumours. The diagnosis of CIS is however often missed when conventional histological techniques are used. No specific immunological marker for CIS germ cells of the testis has been demonstrated previously. A novel monoclonal antibody, M2A, reacting with malignant germ cells of seminomas has recently been developed. Using the immunoperoxidase reaction on tissue sections, we tested the reactivity of M2A with CIS germ cells of the human adult testis. Positive reaction was found in 19 of 20 testicular specimens showing CIS, whereas no staining was found in 39 testicular biopsies without CIS. Thus, M2A may serve as a diagnostic marker in detection of CIS germ cells.
KW - Adult
KW - Antibodies, Monoclonal
KW - Carcinoma in Situ/diagnosis
KW - Humans
KW - Immunoenzyme Techniques
KW - Male
KW - Neoplasms, Germ Cell and Embryonal/diagnosis
KW - Testicular Neoplasms/diagnosis
U2 - 10.1111/j.1699-0463.1988.tb00927.x
DO - 10.1111/j.1699-0463.1988.tb00927.x
M3 - Journal article
C2 - 2843208
SN - 0903-4641
VL - 96
SP - 667
EP - 670
JO - APMIS - Journal of Pathology, Microbiology and Immunology
JF - APMIS - Journal of Pathology, Microbiology and Immunology
IS - 8
ER -