A HPLC method for the simultaneous determination of disopyramide, lidocaine and their monodealkylated metabolites

H R Angelo; J Bonde; J P Kampmann; J Kastrup

doi:10.3109/00365518609083723

A HPLC method for the simultaneous determination of disopyramide, lidocaine and their monodealkylated metabolites

H R Angelo, J Bonde, J P Kampmann, J Kastrup

Abstract

This paper describes a simple high-performance liquid chromatographic method for the determination of disopyramide and lidocaine simultaneously with their dealkylated metabolites. After basic tert-butyl methyl ether extraction and back-extraction with phosphoric acid, the drugs and their metabolites were injected into a Supelcosil CN column and the absorbance of the eluate was measured at 215 nm. The sensitivity was 0.3 mumol/l and the obtained precision, selectivity and stability during storage were adequate for the performed clinical studies in patients therapeutically treated with disopyramide and/or lidocaine. Some results from the clinical studies are briefly presented.

Originalsprog	Engelsk
Tidsskrift	Scandinavian Journal of Clinical and Laboratory Investigation
Vol/bind	46
Udgave nummer	7
Sider (fra-til)	623-7
Antal sider	5
ISSN	0036-5513
DOI	https://doi.org/10.3109/00365518609083723
Status	Udgivet - nov. 1986
Udgivet eksternt	Ja

Adgang til dokumentet

10.3109/00365518609083723

Citationsformater

@article{9744b667672f4173932064145bab445b,

title = "A HPLC method for the simultaneous determination of disopyramide, lidocaine and their monodealkylated metabolites",

abstract = "This paper describes a simple high-performance liquid chromatographic method for the determination of disopyramide and lidocaine simultaneously with their dealkylated metabolites. After basic tert-butyl methyl ether extraction and back-extraction with phosphoric acid, the drugs and their metabolites were injected into a Supelcosil CN column and the absorbance of the eluate was measured at 215 nm. The sensitivity was 0.3 mumol/l and the obtained precision, selectivity and stability during storage were adequate for the performed clinical studies in patients therapeutically treated with disopyramide and/or lidocaine. Some results from the clinical studies are briefly presented.",

keywords = "Chromatography, High Pressure Liquid/methods, Disopyramide/blood, Humans, Lidocaine/blood",

author = "Angelo, {H R} and J Bonde and Kampmann, {J P} and J Kastrup",

year = "1986",

month = nov,

doi = "10.3109/00365518609083723",

language = "English",

volume = "46",

pages = "623--7",

journal = "Scandinavian Journal of Clinical and Laboratory Investigation",

issn = "0036-5513",

publisher = "Informa Healthcare",

number = "7",

}

TY - JOUR

T1 - A HPLC method for the simultaneous determination of disopyramide, lidocaine and their monodealkylated metabolites

AU - Angelo, H R

AU - Bonde, J

AU - Kampmann, J P

AU - Kastrup, J

PY - 1986/11

Y1 - 1986/11

N2 - This paper describes a simple high-performance liquid chromatographic method for the determination of disopyramide and lidocaine simultaneously with their dealkylated metabolites. After basic tert-butyl methyl ether extraction and back-extraction with phosphoric acid, the drugs and their metabolites were injected into a Supelcosil CN column and the absorbance of the eluate was measured at 215 nm. The sensitivity was 0.3 mumol/l and the obtained precision, selectivity and stability during storage were adequate for the performed clinical studies in patients therapeutically treated with disopyramide and/or lidocaine. Some results from the clinical studies are briefly presented.

AB - This paper describes a simple high-performance liquid chromatographic method for the determination of disopyramide and lidocaine simultaneously with their dealkylated metabolites. After basic tert-butyl methyl ether extraction and back-extraction with phosphoric acid, the drugs and their metabolites were injected into a Supelcosil CN column and the absorbance of the eluate was measured at 215 nm. The sensitivity was 0.3 mumol/l and the obtained precision, selectivity and stability during storage were adequate for the performed clinical studies in patients therapeutically treated with disopyramide and/or lidocaine. Some results from the clinical studies are briefly presented.

KW - Chromatography, High Pressure Liquid/methods

KW - Disopyramide/blood

KW - Humans

KW - Lidocaine/blood

U2 - 10.3109/00365518609083723

DO - 10.3109/00365518609083723

M3 - Journal article

C2 - 3787162

SN - 0036-5513

VL - 46

SP - 623

EP - 627

JO - Scandinavian Journal of Clinical and Laboratory Investigation

JF - Scandinavian Journal of Clinical and Laboratory Investigation

IS - 7

ER -

A HPLC method for the simultaneous determination of disopyramide, lidocaine and their monodealkylated metabolites

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater