A European survey on anaerobes from paediatric blood cultures: a comparative analysis with adults

Matteo Boattini; Arianna Aceti; Paulo Bastos; Viktoria Eirini Mavromanolaki; Sofia Maraki; Anastasia Spiliopoulou; Vasileios Kakouris; Yordan Kalchev; Ana Budimir; Branka Bedenić; Zana Rubic; Monica Licker; Corina Musuroi; Emese Juhász; Katalin Kristóf; Mateja Pirs; Ivana Velimirovic; Michael Berktold; Adriána Liptáková; Adriana Krajčíková; Pavel Drevinek; Anezka Gryndlerova; Monika Brzychczy-Wloch; Aldona Olechowska-Jarząb; Filip Bielec; Małgorzata Brauncajs; Edyta Podsiadly; Grażyna Nurzyńska; Patrycja Zalas-Więcek; Anna Michalska; Kristian Riesbeck; Hanna-Sofia Andersson; Tone Tønjum; Johan Christian Berild; Truls Michael Leegaard; Asger Nellemann Rasmussen; Kristian Schønning; Stefan Glöckner; Jürgen Rödel; Mohamed Tarek Badr; Georg Alexander Häcker; Denise Stark; Axel Hamprecht; Anna Dudakova; Jonathan Jantsch; Stefano Mancini; Chantal Quiblier; Damien Jacot; Gilbert Greub; Tiziano Ferniani; Simone Ambretti; Maddalena Calvo; Stefania Stefani; Rogier Schade; Erlangga Yusuf; Stefanie van Kleef-van Koeveringe; Sarah Vandamme; Alexia Verroken; Hector Rodriguez-Villalobos; François Duigou; Stéphane Corvec; Pauline Floch; Clémence Massip; Delphine Chainier; Olivier Barraud; Marie-Bossuète Louissaint; Assaf Mizrahi; Saied Ali; Maeve Doyle; Clarice Egan; Sinead McNicholas; Javier Colomina-Rodriguez; Ignacio Torres; Raul Gillaranz Luengo; Maria Nieves Larrosa Escartín; Maria Belen Viñado Perez; Ana Verónica Halperin; Sergio Fuente García; Rafael Cantón; Miguel Seruca; Vasco Santos Mendes; Dinah Carvalho; Rossana Cavallo; José Melo Cristino; Cristina Costa; Gabriele Bianco

doi:10.1007/s10096-025-05185-7

A European survey on anaerobes from paediatric blood cultures: a comparative analysis with adults

Matteo Boattini^*, Arianna Aceti, Paulo Bastos, Viktoria Eirini Mavromanolaki, Sofia Maraki, Anastasia Spiliopoulou, Vasileios Kakouris, Yordan Kalchev, Ana Budimir, Branka Bedenić, Zana Rubic, Monica Licker, Corina Musuroi, Emese Juhász, Katalin Kristóf, Mateja Pirs, Ivana Velimirovic, Michael Berktold, Adriána Liptáková, Adriana KrajčíkováPavel Drevinek, Anezka Gryndlerova, Monika Brzychczy-Wloch, Aldona Olechowska-Jarząb, Filip Bielec, Małgorzata Brauncajs, Edyta Podsiadly, Grażyna Nurzyńska, Patrycja Zalas-Więcek, Anna Michalska, Kristian Riesbeck, Hanna-Sofia Andersson, Tone Tønjum, Johan Christian Berild, Truls Michael Leegaard, Asger Nellemann Rasmussen, Kristian Schønning, Stefan Glöckner, Jürgen Rödel, Mohamed Tarek Badr, Georg Alexander Häcker, Denise Stark, Axel Hamprecht, Anna Dudakova, Jonathan Jantsch, Stefano Mancini, Chantal Quiblier, Damien Jacot, Gilbert Greub, Tiziano Ferniani, Simone Ambretti, Maddalena Calvo, Stefania Stefani, Rogier Schade, Erlangga Yusuf, Stefanie van Kleef-van Koeveringe, Sarah Vandamme, Alexia Verroken, Hector Rodriguez-Villalobos, François Duigou, Stéphane Corvec, Pauline Floch, Clémence Massip, Delphine Chainier, Olivier Barraud, Marie-Bossuète Louissaint, Assaf Mizrahi, Saied Ali, Maeve Doyle, Clarice Egan, Sinead McNicholas, Javier Colomina-Rodriguez, Ignacio Torres, Raul Gillaranz Luengo, Maria Nieves Larrosa Escartín, Maria Belen Viñado Perez, Ana Verónica Halperin, Sergio Fuente García, Rafael Cantón, Miguel Seruca, Vasco Santos Mendes, Dinah Carvalho, Rossana Cavallo, José Melo Cristino, Cristina Costa, Gabriele Bianco

^*Corresponding author af dette arbejde

Afdeling for Klinisk Mikrobiologi

Abstract

Infections caused by anaerobes are common in children. However, limited data are available on bloodstream infections caused by these bacteria in Europe. A multicentre retrospective observational study was conducted over a 4-year period (2020-2023) across 44 European hospitals to analyze all anaerobes isolated from blood cultures. The study examined the epidemiology and antimicrobial resistance profiles of anaerobes identified in paediatric patients, comparing the findings with those observed in adults. Among the 14,527 total anaerobic isolates, 186 (1.3%) were detected from paediatric patients. These were predominantly Gram-positive (70%) and Gram-negative (22%) bacilli. The most prevalent species in paediatric patients were Cutibacterium acnes (24.7%), Schaalia odontolytica (9.7%), Actinomyces oris (8.1%), and Bacteroides fragilis (7.5%). Relative feature importance based on the mean SHAP (SHapley Additive exPlanations) values distinguished paediatric patients and adults based on their antibiotic resistance patterns with high accuracy. Compared to those from adult patients, Gram-positive bacilli detected in paediatric samples displayed higher resistance rates for meropenem (15% vs. 9%), metronidazole (52% vs. 24%), and vancomycin (27% vs. 6%), and lower resistance to benzylpenicillin (11% vs. 17%), amoxicillin/clavulanate (9% vs. 17%), and clindamycin (30% vs. 36%). Gram-negative bacilli in paediatric samples displayed lower resistance to benzylpenicillin (18% vs. 29%), piperacillin/tazobactam (26% vs. 33%), and clindamycin (20% vs. 27%), and they were highly susceptible to imipenem, meropenem, and metronidazole as those detected in adult patients. Bacteroides species detected in paediatric and adult patients displayed high resistance to piperacillin/tazobactam (33% vs. 39%) and clindamycin (38% vs. 29%), while they were highly susceptible to metronidazole. Compared to those from adult samples, Cutibacterium acnes in paediatric patients displayed lower resistance to benzylpenicillin (none vs. 15%) and clindamycin (17% vs. 29%). The comparison of species and susceptibility profiles of anaerobes detected in paediatric and adult patients highlighted the importance of reporting antimicrobial susceptibility surveillance data by age group.

Originalsprog	Engelsk
Tidsskrift	European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
ISSN	0934-9723
DOI	https://doi.org/10.1007/s10096-025-05185-7
Status	E-pub ahead of print - 19 jun. 2025

Adgang til dokumentet

10.1007/s10096-025-05185-7

Citationsformater

Boattini, M., Aceti, A., Bastos, P., Mavromanolaki, V. E., Maraki, S., Spiliopoulou, A., Kakouris, V., Kalchev, Y., Budimir, A., Bedenić, B., Rubic, Z., Licker, M., Musuroi, C., Juhász, E., Kristóf, K., Pirs, M., Velimirovic, I., Berktold, M., Liptáková, A., ... Bianco, G. (2025). A European survey on anaerobes from paediatric blood cultures: a comparative analysis with adults. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology. Advance online publication. https://doi.org/10.1007/s10096-025-05185-7

A European survey on anaerobes from paediatric blood cultures: a comparative analysis with adults. / Boattini, Matteo; Aceti, Arianna; Bastos, Paulo et al.
I: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 19.06.2025.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Boattini, M, Aceti, A, Bastos, P, Mavromanolaki, VE, Maraki, S, Spiliopoulou, A, Kakouris, V, Kalchev, Y, Budimir, A, Bedenić, B, Rubic, Z, Licker, M, Musuroi, C, Juhász, E, Kristóf, K, Pirs, M, Velimirovic, I, Berktold, M, Liptáková, A, Krajčíková, A, Drevinek, P, Gryndlerova, A, Brzychczy-Wloch, M, Olechowska-Jarząb, A, Bielec, F, Brauncajs, M, Podsiadly, E, Nurzyńska, G, Zalas-Więcek, P, Michalska, A, Riesbeck, K, Andersson, H-S, Tønjum, T, Berild, JC, Leegaard, TM, Rasmussen, AN , Schønning, K, Glöckner, S, Rödel, J, Badr, MT, Häcker, GA, Stark, D, Hamprecht, A, Dudakova, A, Jantsch, J, Mancini, S, Quiblier, C, Jacot, D, Greub, G, Ferniani, T, Ambretti, S, Calvo, M, Stefani, S, Schade, R, Yusuf, E, van Kleef-van Koeveringe, S, Vandamme, S, Verroken, A, Rodriguez-Villalobos, H, Duigou, F, Corvec, S, Floch, P, Massip, C, Chainier, D, Barraud, O, Louissaint, M-B, Mizrahi, A, Ali, S, Doyle, M, Egan, C, McNicholas, S, Colomina-Rodriguez, J, Torres, I, Gillaranz Luengo, R, Larrosa Escartín, MN, Viñado Perez, MB, Halperin, AV, Fuente García, S, Cantón, R, Seruca, M, Santos Mendes, V, Carvalho, D, Cavallo, R, Melo Cristino, J, Costa, C & Bianco, G 2025, 'A European survey on anaerobes from paediatric blood cultures: a comparative analysis with adults', European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology. https://doi.org/10.1007/s10096-025-05185-7

Boattini M, Aceti A, Bastos P, Mavromanolaki VE, Maraki S, Spiliopoulou A et al. A European survey on anaerobes from paediatric blood cultures: a comparative analysis with adults. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology. 2025 jun. 19. Epub 2025 jun. 19. doi: 10.1007/s10096-025-05185-7

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title = "A European survey on anaerobes from paediatric blood cultures: a comparative analysis with adults",

abstract = "Infections caused by anaerobes are common in children. However, limited data are available on bloodstream infections caused by these bacteria in Europe. A multicentre retrospective observational study was conducted over a 4-year period (2020-2023) across 44 European hospitals to analyze all anaerobes isolated from blood cultures. The study examined the epidemiology and antimicrobial resistance profiles of anaerobes identified in paediatric patients, comparing the findings with those observed in adults. Among the 14,527 total anaerobic isolates, 186 (1.3%) were detected from paediatric patients. These were predominantly Gram-positive (70%) and Gram-negative (22%) bacilli. The most prevalent species in paediatric patients were Cutibacterium acnes (24.7%), Schaalia odontolytica (9.7%), Actinomyces oris (8.1%), and Bacteroides fragilis (7.5%). Relative feature importance based on the mean SHAP (SHapley Additive exPlanations) values distinguished paediatric patients and adults based on their antibiotic resistance patterns with high accuracy. Compared to those from adult patients, Gram-positive bacilli detected in paediatric samples displayed higher resistance rates for meropenem (15% vs. 9%), metronidazole (52% vs. 24%), and vancomycin (27% vs. 6%), and lower resistance to benzylpenicillin (11% vs. 17%), amoxicillin/clavulanate (9% vs. 17%), and clindamycin (30% vs. 36%). Gram-negative bacilli in paediatric samples displayed lower resistance to benzylpenicillin (18% vs. 29%), piperacillin/tazobactam (26% vs. 33%), and clindamycin (20% vs. 27%), and they were highly susceptible to imipenem, meropenem, and metronidazole as those detected in adult patients. Bacteroides species detected in paediatric and adult patients displayed high resistance to piperacillin/tazobactam (33% vs. 39%) and clindamycin (38% vs. 29%), while they were highly susceptible to metronidazole. Compared to those from adult samples, Cutibacterium acnes in paediatric patients displayed lower resistance to benzylpenicillin (none vs. 15%) and clindamycin (17% vs. 29%). The comparison of species and susceptibility profiles of anaerobes detected in paediatric and adult patients highlighted the importance of reporting antimicrobial susceptibility surveillance data by age group.",

author = "Matteo Boattini and Arianna Aceti and Paulo Bastos and Mavromanolaki, {Viktoria Eirini} and Sofia Maraki and Anastasia Spiliopoulou and Vasileios Kakouris and Yordan Kalchev and Ana Budimir and Branka Bedeni{\'c} and Zana Rubic and Monica Licker and Corina Musuroi and Emese Juh{\'a}sz and Katalin Krist{\'o}f and Mateja Pirs and Ivana Velimirovic and Michael Berktold and Adri{\'a}na Lipt{\'a}kov{\'a} and Adriana Kraj{\v c}{\'i}kov{\'a} and Pavel Drevinek and Anezka Gryndlerova and Monika Brzychczy-Wloch and Aldona Olechowska-Jarz{\c a}b and Filip Bielec and Ma{\l}gorzata Brauncajs and Edyta Podsiadly and Gra{\.z}yna Nurzy{\'n}ska and Patrycja Zalas-Wi{\c e}cek and Anna Michalska and Kristian Riesbeck and Hanna-Sofia Andersson and Tone T{\o}njum and Berild, {Johan Christian} and Leegaard, {Truls Michael} and Rasmussen, {Asger Nellemann} and Kristian Sch{\o}nning and Stefan Gl{\"o}ckner and J{\"u}rgen R{\"o}del and Badr, {Mohamed Tarek} and H{\"a}cker, {Georg Alexander} and Denise Stark and Axel Hamprecht and Anna Dudakova and Jonathan Jantsch and Stefano Mancini and Chantal Quiblier and Damien Jacot and Gilbert Greub and Tiziano Ferniani and Simone Ambretti and Maddalena Calvo and Stefania Stefani and Rogier Schade and Erlangga Yusuf and {van Kleef-van Koeveringe}, Stefanie and Sarah Vandamme and Alexia Verroken and Hector Rodriguez-Villalobos and Fran{\c c}ois Duigou and St{\'e}phane Corvec and Pauline Floch and Cl{\'e}mence Massip and Delphine Chainier and Olivier Barraud and Marie-Bossu{\`e}te Louissaint and Assaf Mizrahi and Saied Ali and Maeve Doyle and Clarice Egan and Sinead McNicholas and Javier Colomina-Rodriguez and Ignacio Torres and {Gillaranz Luengo}, Raul and {Larrosa Escart{\'i}n}, {Maria Nieves} and {Vi{\~n}ado Perez}, {Maria Belen} and Halperin, {Ana Ver{\'o}nica} and {Fuente Garc{\'i}a}, Sergio and Rafael Cant{\'o}n and Miguel Seruca and {Santos Mendes}, Vasco and Dinah Carvalho and Rossana Cavallo and {Melo Cristino}, Jos{\'e} and Cristina Costa and Gabriele Bianco",

note = "{\textcopyright} 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2025",

month = jun,

day = "19",

doi = "10.1007/s10096-025-05185-7",

language = "English",

journal = "European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology",

issn = "0934-9723",

publisher = "Springer Science and Business Media Deutschland GmbH",

}

TY - JOUR

T1 - A European survey on anaerobes from paediatric blood cultures

T2 - a comparative analysis with adults

AU - Boattini, Matteo

AU - Aceti, Arianna

AU - Bastos, Paulo

AU - Mavromanolaki, Viktoria Eirini

AU - Maraki, Sofia

AU - Spiliopoulou, Anastasia

AU - Kakouris, Vasileios

AU - Kalchev, Yordan

AU - Budimir, Ana

AU - Bedenić, Branka

AU - Rubic, Zana

AU - Licker, Monica

AU - Musuroi, Corina

AU - Juhász, Emese

AU - Kristóf, Katalin

AU - Pirs, Mateja

AU - Velimirovic, Ivana

AU - Berktold, Michael

AU - Liptáková, Adriána

AU - Krajčíková, Adriana

AU - Drevinek, Pavel

AU - Gryndlerova, Anezka

AU - Brzychczy-Wloch, Monika

AU - Olechowska-Jarząb, Aldona

AU - Bielec, Filip

AU - Brauncajs, Małgorzata

AU - Podsiadly, Edyta

AU - Nurzyńska, Grażyna

AU - Zalas-Więcek, Patrycja

AU - Michalska, Anna

AU - Riesbeck, Kristian

AU - Andersson, Hanna-Sofia

AU - Tønjum, Tone

AU - Berild, Johan Christian

AU - Leegaard, Truls Michael

AU - Rasmussen, Asger Nellemann

AU - Schønning, Kristian

AU - Glöckner, Stefan

AU - Rödel, Jürgen

AU - Badr, Mohamed Tarek

AU - Häcker, Georg Alexander

AU - Stark, Denise

AU - Hamprecht, Axel

AU - Dudakova, Anna

AU - Jantsch, Jonathan

AU - Mancini, Stefano

AU - Quiblier, Chantal

AU - Jacot, Damien

AU - Greub, Gilbert

AU - Ferniani, Tiziano

AU - Ambretti, Simone

AU - Calvo, Maddalena

AU - Stefani, Stefania

AU - Schade, Rogier

AU - Yusuf, Erlangga

AU - van Kleef-van Koeveringe, Stefanie

AU - Vandamme, Sarah

AU - Verroken, Alexia

AU - Rodriguez-Villalobos, Hector

AU - Duigou, François

AU - Corvec, Stéphane

AU - Floch, Pauline

AU - Massip, Clémence

AU - Chainier, Delphine

AU - Barraud, Olivier

AU - Louissaint, Marie-Bossuète

AU - Mizrahi, Assaf

AU - Ali, Saied

AU - Doyle, Maeve

AU - Egan, Clarice

AU - McNicholas, Sinead

AU - Colomina-Rodriguez, Javier

AU - Torres, Ignacio

AU - Gillaranz Luengo, Raul

AU - Larrosa Escartín, Maria Nieves

AU - Viñado Perez, Maria Belen

AU - Halperin, Ana Verónica

AU - Fuente García, Sergio

AU - Cantón, Rafael

AU - Seruca, Miguel

AU - Santos Mendes, Vasco

AU - Carvalho, Dinah

AU - Cavallo, Rossana

AU - Melo Cristino, José

AU - Costa, Cristina

AU - Bianco, Gabriele

PY - 2025/6/19

Y1 - 2025/6/19

N2 - Infections caused by anaerobes are common in children. However, limited data are available on bloodstream infections caused by these bacteria in Europe. A multicentre retrospective observational study was conducted over a 4-year period (2020-2023) across 44 European hospitals to analyze all anaerobes isolated from blood cultures. The study examined the epidemiology and antimicrobial resistance profiles of anaerobes identified in paediatric patients, comparing the findings with those observed in adults. Among the 14,527 total anaerobic isolates, 186 (1.3%) were detected from paediatric patients. These were predominantly Gram-positive (70%) and Gram-negative (22%) bacilli. The most prevalent species in paediatric patients were Cutibacterium acnes (24.7%), Schaalia odontolytica (9.7%), Actinomyces oris (8.1%), and Bacteroides fragilis (7.5%). Relative feature importance based on the mean SHAP (SHapley Additive exPlanations) values distinguished paediatric patients and adults based on their antibiotic resistance patterns with high accuracy. Compared to those from adult patients, Gram-positive bacilli detected in paediatric samples displayed higher resistance rates for meropenem (15% vs. 9%), metronidazole (52% vs. 24%), and vancomycin (27% vs. 6%), and lower resistance to benzylpenicillin (11% vs. 17%), amoxicillin/clavulanate (9% vs. 17%), and clindamycin (30% vs. 36%). Gram-negative bacilli in paediatric samples displayed lower resistance to benzylpenicillin (18% vs. 29%), piperacillin/tazobactam (26% vs. 33%), and clindamycin (20% vs. 27%), and they were highly susceptible to imipenem, meropenem, and metronidazole as those detected in adult patients. Bacteroides species detected in paediatric and adult patients displayed high resistance to piperacillin/tazobactam (33% vs. 39%) and clindamycin (38% vs. 29%), while they were highly susceptible to metronidazole. Compared to those from adult samples, Cutibacterium acnes in paediatric patients displayed lower resistance to benzylpenicillin (none vs. 15%) and clindamycin (17% vs. 29%). The comparison of species and susceptibility profiles of anaerobes detected in paediatric and adult patients highlighted the importance of reporting antimicrobial susceptibility surveillance data by age group.

AB - Infections caused by anaerobes are common in children. However, limited data are available on bloodstream infections caused by these bacteria in Europe. A multicentre retrospective observational study was conducted over a 4-year period (2020-2023) across 44 European hospitals to analyze all anaerobes isolated from blood cultures. The study examined the epidemiology and antimicrobial resistance profiles of anaerobes identified in paediatric patients, comparing the findings with those observed in adults. Among the 14,527 total anaerobic isolates, 186 (1.3%) were detected from paediatric patients. These were predominantly Gram-positive (70%) and Gram-negative (22%) bacilli. The most prevalent species in paediatric patients were Cutibacterium acnes (24.7%), Schaalia odontolytica (9.7%), Actinomyces oris (8.1%), and Bacteroides fragilis (7.5%). Relative feature importance based on the mean SHAP (SHapley Additive exPlanations) values distinguished paediatric patients and adults based on their antibiotic resistance patterns with high accuracy. Compared to those from adult patients, Gram-positive bacilli detected in paediatric samples displayed higher resistance rates for meropenem (15% vs. 9%), metronidazole (52% vs. 24%), and vancomycin (27% vs. 6%), and lower resistance to benzylpenicillin (11% vs. 17%), amoxicillin/clavulanate (9% vs. 17%), and clindamycin (30% vs. 36%). Gram-negative bacilli in paediatric samples displayed lower resistance to benzylpenicillin (18% vs. 29%), piperacillin/tazobactam (26% vs. 33%), and clindamycin (20% vs. 27%), and they were highly susceptible to imipenem, meropenem, and metronidazole as those detected in adult patients. Bacteroides species detected in paediatric and adult patients displayed high resistance to piperacillin/tazobactam (33% vs. 39%) and clindamycin (38% vs. 29%), while they were highly susceptible to metronidazole. Compared to those from adult samples, Cutibacterium acnes in paediatric patients displayed lower resistance to benzylpenicillin (none vs. 15%) and clindamycin (17% vs. 29%). The comparison of species and susceptibility profiles of anaerobes detected in paediatric and adult patients highlighted the importance of reporting antimicrobial susceptibility surveillance data by age group.

U2 - 10.1007/s10096-025-05185-7

DO - 10.1007/s10096-025-05185-7

M3 - Journal article

C2 - 40537731

SN - 0934-9723

JO - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

ER -

A European survey on anaerobes from paediatric blood cultures: a comparative analysis with adults

Abstract

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