A direct method for fast three-dimensional serial reconstruction

A Odgaard; K Andersen; F Melsen; H J Gundersen

doi:10.1111/j.1365-2818.1990.tb03038.x

A direct method for fast three-dimensional serial reconstruction

A Odgaard
, K Andersen
, F Melsen
, H J Gundersen

158 Citationer (Scopus)

Abstract

A method for accurate three-dimensional reconstruction of openly connected porous structures is described. The method is based on embedding of a specimen in a contrast coloured epoxy resin and serial sectioning in a standard hard tissue microtome. A PC-based image processing system is used for direct digitization of the cut surface, and by thresholding two-phase images are obtained. The process is fully automated, and about 170 sections can be produced, digitized, dichotomized, and stored per hour. As an example of its applications, the method is used on trabecular bone, which is an anisotropic porous structure.

Originalsprog	Engelsk
Tidsskrift	Journal of Microscopy
Vol/bind	159
Udgave nummer	Pt 3
Sider (fra-til)	335-42
Antal sider	8
ISSN	0022-2720
DOI	https://doi.org/10.1111/j.1365-2818.1990.tb03038.x
Status	Udgivet - sep. 1990
Udgivet eksternt	Ja

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10.1111/j.1365-2818.1990.tb03038.x

Citationsformater

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abstract = "A method for accurate three-dimensional reconstruction of openly connected porous structures is described. The method is based on embedding of a specimen in a contrast coloured epoxy resin and serial sectioning in a standard hard tissue microtome. A PC-based image processing system is used for direct digitization of the cut surface, and by thresholding two-phase images are obtained. The process is fully automated, and about 170 sections can be produced, digitized, dichotomized, and stored per hour. As an example of its applications, the method is used on trabecular bone, which is an anisotropic porous structure.",

keywords = "Bone and Bones/anatomy \& histology, Image Processing, Computer-Assisted, Microtomy",

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T1 - A direct method for fast three-dimensional serial reconstruction

AU - Odgaard, A

AU - Andersen, K

AU - Melsen, F

AU - Gundersen, H J

PY - 1990/9

Y1 - 1990/9

N2 - A method for accurate three-dimensional reconstruction of openly connected porous structures is described. The method is based on embedding of a specimen in a contrast coloured epoxy resin and serial sectioning in a standard hard tissue microtome. A PC-based image processing system is used for direct digitization of the cut surface, and by thresholding two-phase images are obtained. The process is fully automated, and about 170 sections can be produced, digitized, dichotomized, and stored per hour. As an example of its applications, the method is used on trabecular bone, which is an anisotropic porous structure.

AB - A method for accurate three-dimensional reconstruction of openly connected porous structures is described. The method is based on embedding of a specimen in a contrast coloured epoxy resin and serial sectioning in a standard hard tissue microtome. A PC-based image processing system is used for direct digitization of the cut surface, and by thresholding two-phase images are obtained. The process is fully automated, and about 170 sections can be produced, digitized, dichotomized, and stored per hour. As an example of its applications, the method is used on trabecular bone, which is an anisotropic porous structure.

KW - Bone and Bones/anatomy & histology

KW - Image Processing, Computer-Assisted

KW - Microtomy

U2 - 10.1111/j.1365-2818.1990.tb03038.x

DO - 10.1111/j.1365-2818.1990.tb03038.x

M3 - Journal article

C2 - 2243366

SN - 0022-2720

VL - 159

SP - 335

EP - 342

JO - Journal of Microscopy

JF - Journal of Microscopy

IS - Pt 3

ER -

A direct method for fast three-dimensional serial reconstruction

Abstract

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