A clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction

Steen B Laursen; Claus Yding Andersen; Johnny Hindkjaer; Karin Erb

doi:10.1034/j.1600-0412.2003.00297.x

A clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction

Steen B Laursen, Claus Yding Andersen, Johnny Hindkjaer, Karin Erb

2 Citationer (Scopus)

Abstract

OBJECTIVE: To compare a new density gradient medium, SpermFilter, for purifying spermatozoa in assisted reproduction with the more established medium, PureSperm.

DESIGN: Part 1, a multicenter study on 225 semen samples purified using either PureSperm (115 semen samples) or SpermFilter (110 semen samples). Part 2, a retrospective, single center study on a total of 898 assisted reproductive cycles (245 insemination cycles using husband semen, 58 insemination cycles using donor semen and 595 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.

SETTING: Part 1, three fertility clinics in Denmark (two university-affiliated fertility clinics and one private clinic). Part 2, one university-affiliated fertility clinic in Denmark.

MAIN OUTCOME PARAMETERS: Part 1, purity of purified spermatozoa (% motile), motility index and recovery of motile spermatozoa. Part 2, malformation and baby take-home rates (insemination cycles), fertilization, cleavage, implantation, malformation and baby take-home rates (IVF/ICSI cycles).

RESULTS: No statistical differences were observed in any of the parameters investigated.

CONCLUSION: SpermFilter is a valid alternative to PureSperm in assisted reproduction technology (ART).

Originalsprog	Engelsk
Tidsskrift	Acta Obstetricia et Gynecologica Scandinavica
Vol/bind	82
Udgave nummer	10
Sider (fra-til)	929-35
Antal sider	7
ISSN	0001-6349
DOI	https://doi.org/10.1034/j.1600-0412.2003.00297.x
Status	Udgivet - okt. 2003
Udgivet eksternt	Ja

Adgang til dokumentet

10.1034/j.1600-0412.2003.00297.x

Andre filer og links

Link to publication in Scopus

Citationsformater

A clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction. / Laursen, Steen B; Andersen, Claus Yding; Hindkjaer, Johnny et al.
I: Acta Obstetricia et Gynecologica Scandinavica, Bind 82, Nr. 10, 10.2003, s. 929-35.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{878c9167f9bb4282a7619e16f4c54132,

title = "A clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction",

abstract = "OBJECTIVE: To compare a new density gradient medium, SpermFilter, for purifying spermatozoa in assisted reproduction with the more established medium, PureSperm.DESIGN: Part 1, a multicenter study on 225 semen samples purified using either PureSperm (115 semen samples) or SpermFilter (110 semen samples). Part 2, a retrospective, single center study on a total of 898 assisted reproductive cycles (245 insemination cycles using husband semen, 58 insemination cycles using donor semen and 595 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.SETTING: Part 1, three fertility clinics in Denmark (two university-affiliated fertility clinics and one private clinic). Part 2, one university-affiliated fertility clinic in Denmark.MAIN OUTCOME PARAMETERS: Part 1, purity of purified spermatozoa (\% motile), motility index and recovery of motile spermatozoa. Part 2, malformation and baby take-home rates (insemination cycles), fertilization, cleavage, implantation, malformation and baby take-home rates (IVF/ICSI cycles).RESULTS: No statistical differences were observed in any of the parameters investigated.CONCLUSION: SpermFilter is a valid alternative to PureSperm in assisted reproduction technology (ART).",

keywords = "Adult, Cell Separation, Centrifugation, Density Gradient/methods, Denmark, Female, Fertilization in Vitro, Humans, Male, Pregnancy, Reproductive Techniques, Retrospective Studies, Sperm Injections, Intracytoplasmic, Sperm Motility, Spermatozoa",

author = "Laursen, \{Steen B\} and Andersen, \{Claus Yding\} and Johnny Hindkjaer and Karin Erb",

year = "2003",

month = oct,

doi = "10.1034/j.1600-0412.2003.00297.x",

language = "English",

volume = "82",

pages = "929--35",

journal = "Acta Obstetricia et Gynecologica Scandinavica",

issn = "0001-6349",

publisher = "Wiley-Blackwell",

number = "10",

}

TY - JOUR

T1 - A clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction

AU - Laursen, Steen B

AU - Andersen, Claus Yding

AU - Hindkjaer, Johnny

AU - Erb, Karin

PY - 2003/10

Y1 - 2003/10

N2 - OBJECTIVE: To compare a new density gradient medium, SpermFilter, for purifying spermatozoa in assisted reproduction with the more established medium, PureSperm.DESIGN: Part 1, a multicenter study on 225 semen samples purified using either PureSperm (115 semen samples) or SpermFilter (110 semen samples). Part 2, a retrospective, single center study on a total of 898 assisted reproductive cycles (245 insemination cycles using husband semen, 58 insemination cycles using donor semen and 595 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.SETTING: Part 1, three fertility clinics in Denmark (two university-affiliated fertility clinics and one private clinic). Part 2, one university-affiliated fertility clinic in Denmark.MAIN OUTCOME PARAMETERS: Part 1, purity of purified spermatozoa (% motile), motility index and recovery of motile spermatozoa. Part 2, malformation and baby take-home rates (insemination cycles), fertilization, cleavage, implantation, malformation and baby take-home rates (IVF/ICSI cycles).RESULTS: No statistical differences were observed in any of the parameters investigated.CONCLUSION: SpermFilter is a valid alternative to PureSperm in assisted reproduction technology (ART).

AB - OBJECTIVE: To compare a new density gradient medium, SpermFilter, for purifying spermatozoa in assisted reproduction with the more established medium, PureSperm.DESIGN: Part 1, a multicenter study on 225 semen samples purified using either PureSperm (115 semen samples) or SpermFilter (110 semen samples). Part 2, a retrospective, single center study on a total of 898 assisted reproductive cycles (245 insemination cycles using husband semen, 58 insemination cycles using donor semen and 595 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.SETTING: Part 1, three fertility clinics in Denmark (two university-affiliated fertility clinics and one private clinic). Part 2, one university-affiliated fertility clinic in Denmark.MAIN OUTCOME PARAMETERS: Part 1, purity of purified spermatozoa (% motile), motility index and recovery of motile spermatozoa. Part 2, malformation and baby take-home rates (insemination cycles), fertilization, cleavage, implantation, malformation and baby take-home rates (IVF/ICSI cycles).RESULTS: No statistical differences were observed in any of the parameters investigated.CONCLUSION: SpermFilter is a valid alternative to PureSperm in assisted reproduction technology (ART).

KW - Adult

KW - Cell Separation

KW - Centrifugation, Density Gradient/methods

KW - Denmark

KW - Female

KW - Fertilization in Vitro

KW - Humans

KW - Male

KW - Pregnancy

KW - Reproductive Techniques

KW - Retrospective Studies

KW - Sperm Injections, Intracytoplasmic

KW - Sperm Motility

KW - Spermatozoa

UR - https://www.scopus.com/pages/publications/0141649630

U2 - 10.1034/j.1600-0412.2003.00297.x

DO - 10.1034/j.1600-0412.2003.00297.x

M3 - Journal article

C2 - 12956843

SN - 0001-6349

VL - 82

SP - 929

EP - 935

JO - Acta Obstetricia et Gynecologica Scandinavica

JF - Acta Obstetricia et Gynecologica Scandinavica

IS - 10

ER -

A clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction

Abstract

Adgang til dokumentet

Andre filer og links

Fingeraftryk

Citationsformater