TY - JOUR
T1 - A clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction
AU - Laursen, Steen B
AU - Andersen, Claus Yding
AU - Hindkjaer, Johnny
AU - Erb, Karin
PY - 2003/10
Y1 - 2003/10
N2 - OBJECTIVE: To compare a new density gradient medium, SpermFilter, for purifying spermatozoa in assisted reproduction with the more established medium, PureSperm.DESIGN: Part 1, a multicenter study on 225 semen samples purified using either PureSperm (115 semen samples) or SpermFilter (110 semen samples). Part 2, a retrospective, single center study on a total of 898 assisted reproductive cycles (245 insemination cycles using husband semen, 58 insemination cycles using donor semen and 595 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.SETTING: Part 1, three fertility clinics in Denmark (two university-affiliated fertility clinics and one private clinic). Part 2, one university-affiliated fertility clinic in Denmark.MAIN OUTCOME PARAMETERS: Part 1, purity of purified spermatozoa (% motile), motility index and recovery of motile spermatozoa. Part 2, malformation and baby take-home rates (insemination cycles), fertilization, cleavage, implantation, malformation and baby take-home rates (IVF/ICSI cycles).RESULTS: No statistical differences were observed in any of the parameters investigated.CONCLUSION: SpermFilter is a valid alternative to PureSperm in assisted reproduction technology (ART).
AB - OBJECTIVE: To compare a new density gradient medium, SpermFilter, for purifying spermatozoa in assisted reproduction with the more established medium, PureSperm.DESIGN: Part 1, a multicenter study on 225 semen samples purified using either PureSperm (115 semen samples) or SpermFilter (110 semen samples). Part 2, a retrospective, single center study on a total of 898 assisted reproductive cycles (245 insemination cycles using husband semen, 58 insemination cycles using donor semen and 595 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.SETTING: Part 1, three fertility clinics in Denmark (two university-affiliated fertility clinics and one private clinic). Part 2, one university-affiliated fertility clinic in Denmark.MAIN OUTCOME PARAMETERS: Part 1, purity of purified spermatozoa (% motile), motility index and recovery of motile spermatozoa. Part 2, malformation and baby take-home rates (insemination cycles), fertilization, cleavage, implantation, malformation and baby take-home rates (IVF/ICSI cycles).RESULTS: No statistical differences were observed in any of the parameters investigated.CONCLUSION: SpermFilter is a valid alternative to PureSperm in assisted reproduction technology (ART).
KW - Adult
KW - Cell Separation
KW - Centrifugation, Density Gradient/methods
KW - Denmark
KW - Female
KW - Fertilization in Vitro
KW - Humans
KW - Male
KW - Pregnancy
KW - Reproductive Techniques
KW - Retrospective Studies
KW - Sperm Injections, Intracytoplasmic
KW - Sperm Motility
KW - Spermatozoa
U2 - 10.1034/j.1600-0412.2003.00297.x
DO - 10.1034/j.1600-0412.2003.00297.x
M3 - Journal article
C2 - 12956843
SN - 0001-6349
VL - 82
SP - 929
EP - 935
JO - Acta Obstetricia et Gynecologica Scandinavica
JF - Acta Obstetricia et Gynecologica Scandinavica
IS - 10
ER -