TY - JOUR
T1 - 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropism
AU - Adams, Yvonne
AU - Clausen, Anne Skovsbo
AU - Jensen, Peter Østrup
AU - Lager, Malin
AU - Wilhelmsson, Peter
AU - Henningson, Anna J
AU - Lindgren, Per-Eric
AU - Faurholt-Jepsen, Daniel
AU - Mens, Helene
AU - Kraiczy, Peter
AU - Kragh, Kasper Nørskov
AU - Bjarnsholt, Thomas
AU - Kjaer, Andreas
AU - Lebech, Anne-Mette
AU - Jensen, Anja R
N1 - © 2022.
PY - 2023/1/20
Y1 - 2023/1/20
N2 - Lyme neuroborreliosis (LNB), a tick-borne infection caused by spirochetes within the Borrelia burgdorferi sensu lato (s.L.) complex, is among the most prevalent bacterial central nervous system (CNS) infections in Europe and the US. Here we have screened a panel of low-passage B. burgdorferi s.l. isolates using a novel, human-derived 3D blood-brain barrier (BBB)-organoid model. We show that human-derived BBB-organoids support the entry of Borrelia spirochetes, leading to swelling of the organoids and a loss of their structural integrity. The use of the BBB-organoid model highlights the organotropism between B. burgdorferi s.l. genospecies and their ability to cross the BBB contributing to CNS infection.
AB - Lyme neuroborreliosis (LNB), a tick-borne infection caused by spirochetes within the Borrelia burgdorferi sensu lato (s.L.) complex, is among the most prevalent bacterial central nervous system (CNS) infections in Europe and the US. Here we have screened a panel of low-passage B. burgdorferi s.l. isolates using a novel, human-derived 3D blood-brain barrier (BBB)-organoid model. We show that human-derived BBB-organoids support the entry of Borrelia spirochetes, leading to swelling of the organoids and a loss of their structural integrity. The use of the BBB-organoid model highlights the organotropism between B. burgdorferi s.l. genospecies and their ability to cross the BBB contributing to CNS infection.
UR - http://www.scopus.com/inward/record.url?scp=85146463707&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2022.105838
DO - 10.1016/j.isci.2022.105838
M3 - Journal article
C2 - 36686395
VL - 26
SP - 105838
JO - iScience
JF - iScience
SN - 2589-0042
IS - 1
M1 - 105838
ER -