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Region Hovedstaden - en del af Københavns Universitetshospital

Signe Ziir Ingvarsen

Postdoc

Københavns Biocenter, Ole Maaløes Vej 5

2200 København N

Danmark

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Expertise

Summary

Molecular biologist experienced in molecular cancer research, extracellular proteolysis  and therapeutic antibody development.

 

Methodological skills

* Molecular biology, including CRISPR/Cas9 genome editing

* Protein chemistry

* Antibody generation and characterization

* Cell culture (bacteria, yeast and mammalian cells)

* Mouse cancer models 

* Assays for proteolytic activity

* Flow cytometry, confocal microscopy, in vivo imaging

 

 

Primære forskningsområder

Cancer invasion and metastasis

Extracellular matrix degradation

Osteosarcoma (primary bone cancer)

Aktuel forskning

In my current project, we study the role of collagen turnover during osteosarcoma growth, invasion and metastasis. We are specifically interested in the role of the collagen degrading enzyme MT1-MMP and the endocytic collagen receptor uPARAP.

Osteosarcoma is the most common primary tumor of the bone. The disease is characterized by invasive tumor growth, leading to extensive bone destruction, and metastasis to the lungs. For patients presenting with metastatic disease, no treatment providing long-term tumor control is available. In a previous study, we have shown that 1) MT1-MMP and uPARAP are highly expressed in human osteosarcoma samples and 2) uPARAP plays a functional role in osteosarcoma dependent bone destruction.

In the current study, we want to further elucidate the role of MT1-MMP and uPARAP during osteosarcoma progression. For this purpose, we use an orthotopic osteosarcoma mouse model, where the cancer cells are transplanted into the tibia. To allow non-invasive quantification of primary tumor growth and metastasis, we have generated luciferase expressing osteosarcoma cells and demonstrated that both intra-tibial tumor growth and the occurrence of lung metastases can be monitored in vivo by bioluminescent imaging. Furthermore, by utilization of CRISPR/Cas9 mediated genome editing, we have generated a panel of luciferase expressing osteosarcoma cell lines deficient for either MT1-MMP, uPARAP or both. These cell lines will be used in in vivo experiments to study the role of MT1-MMP and uPARAP during osteosarcoma growth, invasion and metastasis. 

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