Research
Print page Print page
Switch language
Bispebjerg Hospital - a part of Copenhagen University Hospital
Published

The effects of 3 weeks of oral glutathione supplementation on whole body insulin sensitivity in obese males with and without type 2 diabetes: A randomized trial

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. The influence of age, sex and cardiorespiratory fitness on maximal fat oxidation rate

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Coenzyme Q10 does not improve peripheral insulin sensitivity in statin-treated men and women; the LIFESTAT study

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Systemic DNA and RNA damage from oxidation after serotonergic treatment of unipolar depression

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Exposure to Tramadol During Early Pregnancy and Risk of Spontaneous Abortion or Major Congenital Malformations

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The effect of oral glutathione (GSH) supplementation was studied in obese subjects with and without type 2 diabetes (T2DM) on measures of glucose homeostasis and markers of oxidative stress. Twenty subjects (10 patients with T2DM and 10 obese subjects) were recruited for the study, and randomized in a double-blinded placebo-controlled manner to consume either 1000 mg GSH per day or placebo for 3 weeks. Before and after the 3 weeks insulin sensitivity was measured with the hyperinsulinemic-euglycemic clamp and a muscle biopsy was obtained to measure GSH and skeletal muscle mitochondrial hydrogen peroxide (H2O2) emission rate. Whole body insulin sensitivity increased significantly in the GSH group. Skeletal muscle GSH was numerically increased (∼19%) in the GSH group; no change was seen in GSH to glutathione disulfide ratio. Skeletal muscle mitochondrial H2O2 emission rate did not change in response to the intervention and neither did the urinary excretion of the RNA oxidation product 8-oxo-7,8-dihydroguanosine or the DNA oxidation product 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), although 8-oxodG decreased as a main effect of time. Oral GSH supplementation improves insulin sensitivity in obese subjects with and without T2DM, although it does not alter markers of oxidative stress. The study has been registered in clinicaltrials.gov (NCT02948673). Novelty: Reduced glutathione supplementation increases insulin sensitivity in obese subjects with and without T2DM. H2O2 emission rate from skeletal muscle mitochondria was not affected by GSH supplementation.

Original languageEnglish
Article number1099
JournalApplied Physiology, Nutrition and Metabolism
Volume46
Issue number9
Pages (from-to)1133-1142
Number of pages10
ISSN1715-5312
DOIs
Publication statusPublished - Sep 2021

    Research areas

  • Glutathione, Insulin sensitivity, Mitochondria, Oxidative stress

ID: 64729695