Research
Print page Print page
Switch language
Bispebjerg Hospital - a part of Copenhagen University Hospital
Published

Stability of the cathelicidin peptide LL-37 in a non-healing wound environment

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Management of Ocular Manifestations of Atopic Dermatitis: A Consensus Meeting Using a Modified Delphi Process

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Clinical Management of Atopic Dermatitis in Adults: Mapping of Expert Opinion in 4 Nordic Countries using a Modified Delphi Process

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Patient-reported Outcomes During Treatment in Patients with Moderate-to-severe Psoriasis: A Danish Nationwide Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Abdominal wall hernias-A local manifestation of systemically impaired quality of the extracellular matrix

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. S100A8/A9 is an important host defence mediator in neuropathic foot ulcers in patients with type 2 diabetes mellitus

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Therapeutic improvement of colonic anastomotic healing under complicated conditions: A systematic review

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Acute Ultraviolet Radiation Perturbs Epithelialization but not the Biomechanical Strength of Full-thickness Cutaneous Wounds

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Wound Healing Biomaterials: Therapies and Regeneration

    Research output: Book/ReportBookResearchpeer-review

View graph of relations
The endogenous cathelicidin peptide LL-37 is strongly expressed at the wound edge early in the process of acute wound healing, but only weakly expressed in chronic wounds. Excessive proteolysis may limit the therapeutic usefulness of exogenous LL-37, especially in ulcers colonized with Pseudomonas aeruginosa that produce elastase, which degrades LL-37. This study investigated the stability of synthetic LL-37 against two types of proteinases in the presence or absence of wound fluid samples (diluted to 10-20%) from nine non-healing venous leg ulcers. Incubation of LL-37 (10 µg/ml) at 37°C for 6 h resulted in complete degradation by the serine proteinase trypsin (≥ 10 ng/ml), while no degradation was observed with matrix metalloproteinase-9. LL-37 susceptibility to trypsin was diminished considerably in the presence of wound fluid, and there was no apparent cleavage of exogenous LL-37 incubated in wound fluid for up to 24 h at 37°C even when using fluids from ulcers with resident P. aeruginosa (n = 2). In conclusion, LL-37 was degraded by trypsin, but not by matrix metalloproteinase-9, and was fairly resistant to proteolytic cleavage ex vivo by incubation with wound fluid from non-healing venous leg ulcers. Thus, the proteolytic environment of chronic wounds does not seem to prevent the therapeutic use of topical LL-37.
Original languageEnglish
JournalActa Dermato Venereologica
Volume91
Issue number5
Pages (from-to)511-5
Number of pages5
ISSN0001-5555
DOIs
Publication statusPublished - 2011

    Research areas

  • Aged, Aged, 80 and over, Antimicrobial Cationic Peptides, Drug Stability, Female, Humans, Leukocyte Elastase, Male, Matrix Metalloproteinase 9, Pseudomonas aeruginosa, Temperature, Time Factors, Trypsin, Varicose Ulcer, Wound Healing

ID: 33248452