Research
Print page Print page
Switch language
Bispebjerg Hospital - a part of Copenhagen University Hospital
Published

Preserved capacity for satellite cell proliferation, regeneration, and hypertrophy in the skeletal muscle of healthy elderly men

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Thyroid hormone receptor α in skeletal muscle is essential for T3-mediated increase in energy expenditure

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Early development of tendinopathy in humans: Sequence of pathological changes in structure and tissue turnover signaling

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Muscle-strain injury exudate favors acute tissue healing and prolonged connective tissue formation in humans

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Carbon-14 bomb pulse dating shows that tendinopathy is preceded by years of abnormally high collagen turnover

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Collagens in primary frozen shoulder: expression of collagen mRNA isoforms in the different phases of the disease

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Distribution of concurrent training sessions does not impact endurance adaptation

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations
Blunted muscle hypertrophy and impaired regeneration with aging have been partly attributed to satellite cell (SC) dysfunction. However, true muscle regeneration has not yet been studied in elderly individuals. To investigate this, muscle injury was induced by 200 electrically stimulated (ES) eccentric contractions of the vastus lateralis (VL) of one leg in seven young (20-31 years) and 19 elderly men (60-73 years). This was followed by 13 weeks of resistance training (RT) for both legs to investigate the capacity for hypertrophy. Muscle biopsies were collected Pre- and Post-RT, and 9 days after ES, for immunohistochemistry and RT-PCR. Hypertrophy was assessed by MRI, DEXA, and immunohistochemistry. Overall, surprisingly comparable responses were observed between the young and elderly. Nine days after ES, Pax7+ SC number had doubled (P < .05), alongside necrosis and substantial changes in expression of genes related to matrix, myogenesis, and innervation (P < .05). Post-RT, VL cross-sectional area had increased in both legs (~15%, P < .05) and SCs/type II fiber had increased ~2-4 times more with ES+RT vs RT alone (P < .001). Together these novel findings demonstrate "youthful" regeneration and hypertrophy responses in human elderly muscle. Furthermore, boosting SC availability in healthy elderly men does not enhance the subsequent muscle hypertrophy response to RT. Keywords: Sarcopenia; heavy resistance training; human; in vivo myogenesis; myofiber necrosis; myogenic progenitor cells.
Original languageEnglish
JournalFASEB Journal
ISSN0892-6638
Publication statusPublished - May 2020

ID: 61757572