Research
Print page Print page
Switch language
Bispebjerg Hospital - a part of Copenhagen University Hospital
Published

Non-invasive measurement of reepithelialization and microvascularity of suction-blister wounds with benchmarking to histology

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Corrigendum to "Large Incisional Hernias Increase in Size" [Journal of Surgical Research 2019; 244: 160-165.]

    Research output: Contribution to journalComment/debateResearchpeer-review

  2. SARS-CoV-2 infection and venous thromboembolism after surgery: an international prospective cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Impact of the COVID-19 pandemic on the management of colorectal cancer in Denmark

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. The wound healing effect of local leukocyte platelet-rich plasma after total hip arthroplasty: A randomized controlled trial

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

We explored use of the suction-blister wound model in the assessment of not only epidermal regeneration but also pain, the microvascular response and bacteriology. The effects of topical zinc sulfate were studied to articulate the methodologies in this double-blind trial. One epidermal suction blister (10 mm) was induced on each buttock in 30 healthy volunteers (15 females:15 males) and de-roofed on day 0. The wounds were randomized to daily treatment with 1.4% zinc sulfate shower gel (n = 20), placebo (n = 20) or control (n = 20). Digital photography coupled with planimetry, transepidermal water loss (TEWL) measurement and optical coherence tomography (OCT) was benchmarked to the gold standard of histology of 60 full-thickness wound biopsies on day 4. Pain increased after application of the shower gels. Vessel density, determined from OCT images, increased from day 0 to day 2 in the three groups but increased more with the placebo than with the zinc shower gel (p = 0.003) or the control treatment (p = 0.002) and correlated (rS = 0.313, p = 0.015) with the inflammatory response on day 4, as determined by histology. Coagulase-negative staphylococci were more common in wounds compared with skin (p = 0.002) and was reduced (p = 0.030) with zinc sulfate treatment. Planimetric analysis of digital wound images was not biased (p = 0.234) compared with histology, and TEWL measurements showed no correlation (rS = 0.052, p = 0.691) with epithelialization. Neoepidermal formation, determined by histology, did not differ (p = 0.290) among the groups. Zinc sulfate reduced (p = 0.031) the release of lactate dehydrogenase from cultured keratinocytes isolated from the blister roofs. Therefore, combination of the standardized suction-blister wound model with non-invasive planimetry and OCT is a useful tool for assessing wound therapies. Zinc sulfate transiently dampened inflammation and reduced bacterial growth. This article is protected by copyright. All rights reserved.

Original languageEnglish
JournalWound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
Volume25
Issue number6
Pages (from-to)984-993
ISSN1067-1927
DOIs
Publication statusPublished - 22 Nov 2017

    Research areas

  • Journal Article

ID: 52541156