Harvard
Hu, T, Buus, TB, Krejsgaard, T, Nansen, A, Lundholt, BK, Spee, P, Fredholm, S, Petersen, DL, Blümel, E, Gluud, M, Monteiro, MN, Willerslev-Olsen, A, Andersen, MH, Straten, PT, Met, Ö, Stolearenco, V
, Fogh, H, Gniadecki, R, Nastasi, C, Litman, T, Woetmann, A, Gjerdrum, LMR & Ødum, N 2019, '
Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome'
Oncotarget, vol. 10, no. 47, pp. 4894-4906.
https://doi.org/10.18632/oncotarget.27122APA
Hu, T., Buus, T. B., Krejsgaard, T., Nansen, A., Lundholt, B. K., Spee, P., ... Ødum, N. (2019).
Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome.
Oncotarget,
10(47), 4894-4906.
https://doi.org/10.18632/oncotarget.27122CBE
Hu T, Buus TB, Krejsgaard T, Nansen A, Lundholt BK, Spee P, Fredholm S, Petersen DL, Blümel E, Gluud M, Monteiro MN, Willerslev-Olsen A, Andersen MH, Straten PT, Met Ö, Stolearenco V
, Fogh H, Gniadecki R, Nastasi C, Litman T, Woetmann A, Gjerdrum LMR, Ødum N. 2019.
Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome.
Oncotarget. 10(47):4894-4906.
https://doi.org/10.18632/oncotarget.27122MLA
Vancouver
Author
Hu, Tengpeng ; Buus, Terkild Brink ; Krejsgaard, Thorbjørn ; Nansen, Anneline ; Lundholt, Betina Kerstin ; Spee, Pieter ; Fredholm, Simon ; Petersen, David Leander ; Blümel, Edda ; Gluud, Maria ; Monteiro, Madalena N ; Willerslev-Olsen, Andreas ; Andersen, Mads Hald ; Straten, Per Thor ; Met, Özcan ; Stolearenco, Veronica
; Fogh, Hanne ; Gniadecki, Robert ; Nastasi, Claudia ; Litman, Thomas ; Woetmann, Anders ; Gjerdrum, Lise Mette Rahbek ; Ødum, Niels. /
Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome. In:
Oncotarget. 2019 ; Vol. 10, No. 47. pp. 4894-4906.
Bibtex
@article{5e28231abfa0456692f4dc3f9230c3eb,
title = "Expression and function of Kv1.3 channel in malignant T cells in S{\'e}zary syndrome",
abstract = "The voltage-gated potassium channel Kv1.3 (KCNA3) is expressed by a subset of chronically activated memory T cells and plays an important role in their activation and proliferation. Here, we show that primary malignant T cells isolated from patients with S{\'e}zary syndrome (SS) express Kv1.3 and are sensitive to potent Kv1.3 inhibitors ShK and Vm24, but not sensitive to a less potent inhibitor [N17A/F32T]-AnTx. Kv1.3 blockade inhibits CD3/CD28-induced proliferation and IL-9 expression by SS cells in a concentration-dependent manner. In parallel, CD3/CD28-mediated CD25 induction is inhibited, whereas Kv1.3 blockade has no effect on apoptosis or cell death as judged by Annexin V and PI staining. In conclusion, we provide the first evidence that malignant T cells in SS express functional Kv1.3 channels and that Kv1.3 blockade inhibits activation-induced proliferation as well as cytokine and cytokine receptor expression in malignant T cells, suggesting that Kv1.3 is a potential target for therapy in SS.",
author = "Tengpeng Hu and Buus, {Terkild Brink} and Thorbj{\o}rn Krejsgaard and Anneline Nansen and Lundholt, {Betina Kerstin} and Pieter Spee and Simon Fredholm and Petersen, {David Leander} and Edda Bl{\"u}mel and Maria Gluud and Monteiro, {Madalena N} and Andreas Willerslev-Olsen and Andersen, {Mads Hald} and Straten, {Per Thor} and {\"O}zcan Met and Veronica Stolearenco and Hanne Fogh and Robert Gniadecki and Claudia Nastasi and Thomas Litman and Anders Woetmann and Gjerdrum, {Lise Mette Rahbek} and Niels {\O}dum",
year = "2019",
month = "8",
day = "6",
doi = "10.18632/oncotarget.27122",
language = "English",
volume = "10",
pages = "4894--4906",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "47",
}
RIS
TY - JOUR
T1 - Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome
AU - Hu, Tengpeng
AU - Buus, Terkild Brink
AU - Krejsgaard, Thorbjørn
AU - Nansen, Anneline
AU - Lundholt, Betina Kerstin
AU - Spee, Pieter
AU - Fredholm, Simon
AU - Petersen, David Leander
AU - Blümel, Edda
AU - Gluud, Maria
AU - Monteiro, Madalena N
AU - Willerslev-Olsen, Andreas
AU - Andersen, Mads Hald
AU - Straten, Per Thor
AU - Met, Özcan
AU - Stolearenco, Veronica
AU - Fogh, Hanne
AU - Gniadecki, Robert
AU - Nastasi, Claudia
AU - Litman, Thomas
AU - Woetmann, Anders
AU - Gjerdrum, Lise Mette Rahbek
AU - Ødum, Niels
PY - 2019/8/6
Y1 - 2019/8/6
N2 - The voltage-gated potassium channel Kv1.3 (KCNA3) is expressed by a subset of chronically activated memory T cells and plays an important role in their activation and proliferation. Here, we show that primary malignant T cells isolated from patients with Sézary syndrome (SS) express Kv1.3 and are sensitive to potent Kv1.3 inhibitors ShK and Vm24, but not sensitive to a less potent inhibitor [N17A/F32T]-AnTx. Kv1.3 blockade inhibits CD3/CD28-induced proliferation and IL-9 expression by SS cells in a concentration-dependent manner. In parallel, CD3/CD28-mediated CD25 induction is inhibited, whereas Kv1.3 blockade has no effect on apoptosis or cell death as judged by Annexin V and PI staining. In conclusion, we provide the first evidence that malignant T cells in SS express functional Kv1.3 channels and that Kv1.3 blockade inhibits activation-induced proliferation as well as cytokine and cytokine receptor expression in malignant T cells, suggesting that Kv1.3 is a potential target for therapy in SS.
AB - The voltage-gated potassium channel Kv1.3 (KCNA3) is expressed by a subset of chronically activated memory T cells and plays an important role in their activation and proliferation. Here, we show that primary malignant T cells isolated from patients with Sézary syndrome (SS) express Kv1.3 and are sensitive to potent Kv1.3 inhibitors ShK and Vm24, but not sensitive to a less potent inhibitor [N17A/F32T]-AnTx. Kv1.3 blockade inhibits CD3/CD28-induced proliferation and IL-9 expression by SS cells in a concentration-dependent manner. In parallel, CD3/CD28-mediated CD25 induction is inhibited, whereas Kv1.3 blockade has no effect on apoptosis or cell death as judged by Annexin V and PI staining. In conclusion, we provide the first evidence that malignant T cells in SS express functional Kv1.3 channels and that Kv1.3 blockade inhibits activation-induced proliferation as well as cytokine and cytokine receptor expression in malignant T cells, suggesting that Kv1.3 is a potential target for therapy in SS.
U2 - 10.18632/oncotarget.27122
DO - 10.18632/oncotarget.27122
M3 - Journal article
VL - 10
SP - 4894
EP - 4906
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
IS - 47
ER -
