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Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome

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Harvard

Hu, T, Buus, TB, Krejsgaard, T, Nansen, A, Lundholt, BK, Spee, P, Fredholm, S, Petersen, DL, Blümel, E, Gluud, M, Monteiro, MN, Willerslev-Olsen, A, Andersen, MH, Straten, PT, Met, Ö, Stolearenco, V, Fogh, H, Gniadecki, R, Nastasi, C, Litman, T, Woetmann, A, Gjerdrum, LMR & Ødum, N 2019, 'Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome' Oncotarget, vol. 10, no. 47, pp. 4894-4906. https://doi.org/10.18632/oncotarget.27122

APA

Hu, T., Buus, T. B., Krejsgaard, T., Nansen, A., Lundholt, B. K., Spee, P., ... Ødum, N. (2019). Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome. Oncotarget, 10(47), 4894-4906. https://doi.org/10.18632/oncotarget.27122

CBE

Hu T, Buus TB, Krejsgaard T, Nansen A, Lundholt BK, Spee P, Fredholm S, Petersen DL, Blümel E, Gluud M, Monteiro MN, Willerslev-Olsen A, Andersen MH, Straten PT, Met Ö, Stolearenco V, Fogh H, Gniadecki R, Nastasi C, Litman T, Woetmann A, Gjerdrum LMR, Ødum N. 2019. Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome. Oncotarget. 10(47):4894-4906. https://doi.org/10.18632/oncotarget.27122

MLA

Vancouver

Hu T, Buus TB, Krejsgaard T, Nansen A, Lundholt BK, Spee P et al. Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome. Oncotarget. 2019 Aug 6;10(47):4894-4906. https://doi.org/10.18632/oncotarget.27122

Author

Hu, Tengpeng ; Buus, Terkild Brink ; Krejsgaard, Thorbjørn ; Nansen, Anneline ; Lundholt, Betina Kerstin ; Spee, Pieter ; Fredholm, Simon ; Petersen, David Leander ; Blümel, Edda ; Gluud, Maria ; Monteiro, Madalena N ; Willerslev-Olsen, Andreas ; Andersen, Mads Hald ; Straten, Per Thor ; Met, Özcan ; Stolearenco, Veronica ; Fogh, Hanne ; Gniadecki, Robert ; Nastasi, Claudia ; Litman, Thomas ; Woetmann, Anders ; Gjerdrum, Lise Mette Rahbek ; Ødum, Niels. / Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome. In: Oncotarget. 2019 ; Vol. 10, No. 47. pp. 4894-4906.

Bibtex

@article{5e28231abfa0456692f4dc3f9230c3eb,
title = "Expression and function of Kv1.3 channel in malignant T cells in S{\'e}zary syndrome",
abstract = "The voltage-gated potassium channel Kv1.3 (KCNA3) is expressed by a subset of chronically activated memory T cells and plays an important role in their activation and proliferation. Here, we show that primary malignant T cells isolated from patients with S{\'e}zary syndrome (SS) express Kv1.3 and are sensitive to potent Kv1.3 inhibitors ShK and Vm24, but not sensitive to a less potent inhibitor [N17A/F32T]-AnTx. Kv1.3 blockade inhibits CD3/CD28-induced proliferation and IL-9 expression by SS cells in a concentration-dependent manner. In parallel, CD3/CD28-mediated CD25 induction is inhibited, whereas Kv1.3 blockade has no effect on apoptosis or cell death as judged by Annexin V and PI staining. In conclusion, we provide the first evidence that malignant T cells in SS express functional Kv1.3 channels and that Kv1.3 blockade inhibits activation-induced proliferation as well as cytokine and cytokine receptor expression in malignant T cells, suggesting that Kv1.3 is a potential target for therapy in SS.",
author = "Tengpeng Hu and Buus, {Terkild Brink} and Thorbj{\o}rn Krejsgaard and Anneline Nansen and Lundholt, {Betina Kerstin} and Pieter Spee and Simon Fredholm and Petersen, {David Leander} and Edda Bl{\"u}mel and Maria Gluud and Monteiro, {Madalena N} and Andreas Willerslev-Olsen and Andersen, {Mads Hald} and Straten, {Per Thor} and {\"O}zcan Met and Veronica Stolearenco and Hanne Fogh and Robert Gniadecki and Claudia Nastasi and Thomas Litman and Anders Woetmann and Gjerdrum, {Lise Mette Rahbek} and Niels {\O}dum",
year = "2019",
month = "8",
day = "6",
doi = "10.18632/oncotarget.27122",
language = "English",
volume = "10",
pages = "4894--4906",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "47",

}

RIS

TY - JOUR

T1 - Expression and function of Kv1.3 channel in malignant T cells in Sézary syndrome

AU - Hu, Tengpeng

AU - Buus, Terkild Brink

AU - Krejsgaard, Thorbjørn

AU - Nansen, Anneline

AU - Lundholt, Betina Kerstin

AU - Spee, Pieter

AU - Fredholm, Simon

AU - Petersen, David Leander

AU - Blümel, Edda

AU - Gluud, Maria

AU - Monteiro, Madalena N

AU - Willerslev-Olsen, Andreas

AU - Andersen, Mads Hald

AU - Straten, Per Thor

AU - Met, Özcan

AU - Stolearenco, Veronica

AU - Fogh, Hanne

AU - Gniadecki, Robert

AU - Nastasi, Claudia

AU - Litman, Thomas

AU - Woetmann, Anders

AU - Gjerdrum, Lise Mette Rahbek

AU - Ødum, Niels

PY - 2019/8/6

Y1 - 2019/8/6

N2 - The voltage-gated potassium channel Kv1.3 (KCNA3) is expressed by a subset of chronically activated memory T cells and plays an important role in their activation and proliferation. Here, we show that primary malignant T cells isolated from patients with Sézary syndrome (SS) express Kv1.3 and are sensitive to potent Kv1.3 inhibitors ShK and Vm24, but not sensitive to a less potent inhibitor [N17A/F32T]-AnTx. Kv1.3 blockade inhibits CD3/CD28-induced proliferation and IL-9 expression by SS cells in a concentration-dependent manner. In parallel, CD3/CD28-mediated CD25 induction is inhibited, whereas Kv1.3 blockade has no effect on apoptosis or cell death as judged by Annexin V and PI staining. In conclusion, we provide the first evidence that malignant T cells in SS express functional Kv1.3 channels and that Kv1.3 blockade inhibits activation-induced proliferation as well as cytokine and cytokine receptor expression in malignant T cells, suggesting that Kv1.3 is a potential target for therapy in SS.

AB - The voltage-gated potassium channel Kv1.3 (KCNA3) is expressed by a subset of chronically activated memory T cells and plays an important role in their activation and proliferation. Here, we show that primary malignant T cells isolated from patients with Sézary syndrome (SS) express Kv1.3 and are sensitive to potent Kv1.3 inhibitors ShK and Vm24, but not sensitive to a less potent inhibitor [N17A/F32T]-AnTx. Kv1.3 blockade inhibits CD3/CD28-induced proliferation and IL-9 expression by SS cells in a concentration-dependent manner. In parallel, CD3/CD28-mediated CD25 induction is inhibited, whereas Kv1.3 blockade has no effect on apoptosis or cell death as judged by Annexin V and PI staining. In conclusion, we provide the first evidence that malignant T cells in SS express functional Kv1.3 channels and that Kv1.3 blockade inhibits activation-induced proliferation as well as cytokine and cytokine receptor expression in malignant T cells, suggesting that Kv1.3 is a potential target for therapy in SS.

U2 - 10.18632/oncotarget.27122

DO - 10.18632/oncotarget.27122

M3 - Journal article

VL - 10

SP - 4894

EP - 4906

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 47

ER -

ID: 58521676