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Bispebjerg Hospital - a part of Copenhagen University Hospital
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Effects of topical corticosteroid and tacrolimus on ceramides and irritancy to sodium lauryl sulphate in healthy skin

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DOI

  1. Management of Ocular Manifestations of Atopic Dermatitis: A Consensus Meeting Using a Modified Delphi Process

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Clinical Management of Atopic Dermatitis in Adults: Mapping of Expert Opinion in 4 Nordic Countries using a Modified Delphi Process

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  3. Patient-reported Outcomes During Treatment in Patients with Moderate-to-severe Psoriasis: A Danish Nationwide Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Management of Ocular Manifestations of Atopic Dermatitis: A Consensus Meeting Using a Modified Delphi Process

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Atopic dermatitis is associated with increased use of social benefits: a register-based cohort study

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  3. The epidemic of methylisothiazolinone contact allergy in Europe: follow-up on changing exposures

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  4. Risk of work-related hand eczema in relation to wet work exposure

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  5. Factors influencing the prognosis for occupational hand eczema - new trends: new trends

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The skin barrier, located in the stratum corneum, is influenced mainly by the lipid and protein composition of this layer. In eczematous diseases impairment of the skin barrier is thought to be of prime importance. Topical anti-inflammatory drugs and emollients are the most widely used eczema treatments. The aim of this study was to examine the effects of topically applied corticosteroid, tacrolimus and emollient on stratum corneum lipids and barrier parameters. Nineteen healthy volunteers participated in the study. Both forearms of the subjects were divided into four areas, which were treated twice daily for one week with betamethasone, tacrolimus, emollient, or left untreated, respectively. After one week each area was challenged with a 24 h sodium lauryl sulphate patch test. The lipids were collected using the cyanoacrylate method and evaluated by high performance thin layer chromatography. For evaluation of the skin barrier, transepidermal water loss, erythema and electrical capacitance were measured. The ceramide/cholesterol ratio was increased in betamethasone- (p = 0.008) and tacrolimus-treated (p = 0.025) skin compared with emollient-treated skin. No differences in ceramide subgroups were found between treatment regimes. Pretreatment with betamethasone (p = 0.01) or with tacrolimus (p = 0.001) causes a decreased inflammatory response to sodium lauryl sulphate compared with emollient. In conclusion, treatment with betamethasone and tacrolimus has a positive effect on the ceramide/cholesterol ratio and susceptibility to irritant reaction compared with an emollient.
Original languageEnglish
JournalActa Dermato Venereologica
Volume91
Issue number3
Pages (from-to)290-4
Number of pages5
ISSN0001-5555
DOIs
Publication statusPublished - 2011

    Research areas

  • Administration, Topical, Adolescent, Adrenal Cortex Hormones, Adult, Betamethasone, Ceramides, Cholesterol, Chromatography, High Pressure Liquid, Denmark, Dermatitis, Irritant, Electric Capacitance, Emollients, Erythema, Female, Forearm, Humans, Immunosuppressive Agents, Male, Middle Aged, Skin, Skin Irritancy Tests, Sodium Dodecyl Sulfate, Tacrolimus, Time Factors, Water Loss, Insensible, Young Adult

ID: 34709562