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Collagens in primary frozen shoulder: expression of collagen mRNA isoforms in the different phases of the disease

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@article{1d2db1b4bbdf40ed9891f91878e75d40,
title = "Collagens in primary frozen shoulder: expression of collagen mRNA isoforms in the different phases of the disease",
abstract = "OBJECTIVES: Primary frozen shoulder (pFS) has three phases that differ in clinical presentation. It is characterized by contracture of the joint capsule. We hypothesized that there is a general upregulation of collagens in pFS, and that this is highest in the first phase of the disease. The aims of this study were to investigate the expression of various collagens and degradation of collagens in patients with primary pFS and relate this to the three phases of the condition.METHODS: From twenty-six patients with pFS and eight control patients with subacromial impingement, biopsies were obtained during shoulder arthroscopy from the middle glenohumeral ligament and the anterior capsule, and mRNA levels for collagens, MMP-2 and -14 and TGF-β1, - β2 and -β3 in the tissue were analysed using real-time PCR.RESULTS: Genes for collagens type I, III, IV, V, VI and XIV, were activated in pFS, and the total mRNA for all collagens was increased (P < 0.05). This upregulation was independent of disease phases in pFS. In addition, MMP-2, MMP-14, TGF-β1 and TGF-β3 were upregulated in all phases of the disease.CONCLUSION: There is a general upregulation and an increased degradation of collagens in pFS in all three phases of the disease. This indicates a constantly increased turnover of the fibrotic tissue in the capsule from pFS. The difference in clinical presentation of pFS observed in the three phases of the disease is not primarily a result of variations in collagen production.",
keywords = "Adult, Biopsy, Bursitis/genetics, Case-Control Studies, Collagen Type I/genetics, Collagen Type III/genetics, Collagen Type IV/genetics, Collagen Type V/genetics, Collagen Type VI/genetics, Collagen/genetics, Disease Progression, Female, Gene Expression, Humans, Joint Capsule/metabolism, Ligaments/metabolism, Male, Matrix Metalloproteinase 14/genetics, Matrix Metalloproteinase 2/genetics, Middle Aged, RNA, Messenger/metabolism, Transforming Growth Factor beta1/genetics, Transforming Growth Factor beta2/genetics, Transforming Growth Factor beta3/genetics, Up-Regulation, collagen turnover, fibroblasts, disease activity, frozen shoulder",
author = "Line Marker and Peter Schjerling and Mackey, {Abigail L} and Thomas Hansen and Jens Jakobsen and Michael Kj{\ae}r and Krogsgaard, {Michael R}",
note = "{\textcopyright} The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.com.",
year = "2021",
month = aug,
day = "2",
doi = "10.1093/rheumatology/keaa802",
language = "English",
volume = "60",
pages = "3879--3887",
journal = "Rheumatology",
issn = "1462-0324",
publisher = "Oxford University Press",
number = "8",

}

RIS

TY - JOUR

T1 - Collagens in primary frozen shoulder

T2 - expression of collagen mRNA isoforms in the different phases of the disease

AU - Marker, Line

AU - Schjerling, Peter

AU - Mackey, Abigail L

AU - Hansen, Thomas

AU - Jakobsen, Jens

AU - Kjær, Michael

AU - Krogsgaard, Michael R

N1 - © The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

PY - 2021/8/2

Y1 - 2021/8/2

N2 - OBJECTIVES: Primary frozen shoulder (pFS) has three phases that differ in clinical presentation. It is characterized by contracture of the joint capsule. We hypothesized that there is a general upregulation of collagens in pFS, and that this is highest in the first phase of the disease. The aims of this study were to investigate the expression of various collagens and degradation of collagens in patients with primary pFS and relate this to the three phases of the condition.METHODS: From twenty-six patients with pFS and eight control patients with subacromial impingement, biopsies were obtained during shoulder arthroscopy from the middle glenohumeral ligament and the anterior capsule, and mRNA levels for collagens, MMP-2 and -14 and TGF-β1, - β2 and -β3 in the tissue were analysed using real-time PCR.RESULTS: Genes for collagens type I, III, IV, V, VI and XIV, were activated in pFS, and the total mRNA for all collagens was increased (P < 0.05). This upregulation was independent of disease phases in pFS. In addition, MMP-2, MMP-14, TGF-β1 and TGF-β3 were upregulated in all phases of the disease.CONCLUSION: There is a general upregulation and an increased degradation of collagens in pFS in all three phases of the disease. This indicates a constantly increased turnover of the fibrotic tissue in the capsule from pFS. The difference in clinical presentation of pFS observed in the three phases of the disease is not primarily a result of variations in collagen production.

AB - OBJECTIVES: Primary frozen shoulder (pFS) has three phases that differ in clinical presentation. It is characterized by contracture of the joint capsule. We hypothesized that there is a general upregulation of collagens in pFS, and that this is highest in the first phase of the disease. The aims of this study were to investigate the expression of various collagens and degradation of collagens in patients with primary pFS and relate this to the three phases of the condition.METHODS: From twenty-six patients with pFS and eight control patients with subacromial impingement, biopsies were obtained during shoulder arthroscopy from the middle glenohumeral ligament and the anterior capsule, and mRNA levels for collagens, MMP-2 and -14 and TGF-β1, - β2 and -β3 in the tissue were analysed using real-time PCR.RESULTS: Genes for collagens type I, III, IV, V, VI and XIV, were activated in pFS, and the total mRNA for all collagens was increased (P < 0.05). This upregulation was independent of disease phases in pFS. In addition, MMP-2, MMP-14, TGF-β1 and TGF-β3 were upregulated in all phases of the disease.CONCLUSION: There is a general upregulation and an increased degradation of collagens in pFS in all three phases of the disease. This indicates a constantly increased turnover of the fibrotic tissue in the capsule from pFS. The difference in clinical presentation of pFS observed in the three phases of the disease is not primarily a result of variations in collagen production.

KW - Adult

KW - Biopsy

KW - Bursitis/genetics

KW - Case-Control Studies

KW - Collagen Type I/genetics

KW - Collagen Type III/genetics

KW - Collagen Type IV/genetics

KW - Collagen Type V/genetics

KW - Collagen Type VI/genetics

KW - Collagen/genetics

KW - Disease Progression

KW - Female

KW - Gene Expression

KW - Humans

KW - Joint Capsule/metabolism

KW - Ligaments/metabolism

KW - Male

KW - Matrix Metalloproteinase 14/genetics

KW - Matrix Metalloproteinase 2/genetics

KW - Middle Aged

KW - RNA, Messenger/metabolism

KW - Transforming Growth Factor beta1/genetics

KW - Transforming Growth Factor beta2/genetics

KW - Transforming Growth Factor beta3/genetics

KW - Up-Regulation

KW - collagen turnover

KW - fibroblasts

KW - disease activity

KW - frozen shoulder

U2 - 10.1093/rheumatology/keaa802

DO - 10.1093/rheumatology/keaa802

M3 - Journal article

C2 - 33347577

VL - 60

SP - 3879

EP - 3887

JO - Rheumatology

JF - Rheumatology

SN - 1462-0324

IS - 8

ER -

ID: 62410830