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Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control

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Harvard

Gallina, I, Colding, C, Henriksen, P, Beli, P, Nakamura, K, Offman, J, Mathiasen, DP, Silva, S, Hoffmann, E, Groth, A, Choudhary, C & Lisby, M 2015, 'Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control', Nature Communications, vol. 6, pp. 6533. https://doi.org/10.1038/ncomms7533

APA

Gallina, I., Colding, C., Henriksen, P., Beli, P., Nakamura, K., Offman, J., Mathiasen, D. P., Silva, S., Hoffmann, E., Groth, A., Choudhary, C., & Lisby, M. (2015). Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control. Nature Communications, 6, 6533. https://doi.org/10.1038/ncomms7533

CBE

Gallina I, Colding C, Henriksen P, Beli P, Nakamura K, Offman J, Mathiasen DP, Silva S, Hoffmann E, Groth A, Choudhary C, Lisby M. 2015. Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control. Nature Communications. 6:6533. https://doi.org/10.1038/ncomms7533

MLA

Vancouver

Author

Gallina, Irene ; Colding, Camilla ; Henriksen, Peter ; Beli, Petra ; Nakamura, Kyosuke ; Offman, Judith ; Mathiasen, David P ; Silva, Sonia ; Hoffmann, Eva ; Groth, Anja ; Choudhary, Chunaram ; Lisby, Michael. / Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control. In: Nature Communications. 2015 ; Vol. 6. pp. 6533.

Bibtex

@article{0eab10601b044957b3a93702746963b4,
title = "Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control",
abstract = "DNA replication stress is a source of genomic instability. Here we identify changed mutation rate 1 (Cmr1) as a factor involved in the response to DNA replication stress in Saccharomyces cerevisiae and show that Cmr1--together with Mrc1/Claspin, Pph3, the chaperonin containing TCP1 (CCT) and 25 other proteins--define a novel intranuclear quality control compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated proteins in response to genotoxic stress. The diversity of proteins that localize to INQ indicates that other biological processes such as cell cycle progression, chromatin and mitotic spindle organization may also be regulated through INQ. Similar to Cmr1, its human orthologue WDR76 responds to proteasome inhibition and DNA damage by relocalizing to nuclear foci and physically associating with CCT, suggesting an evolutionarily conserved biological function. We propose that Cmr1/WDR76 plays a role in the recovery from genotoxic stress through regulation of the turnover of sumoylated and phosphorylated proteins.",
keywords = "Amino Acid Transport Systems/metabolism, Cell Cycle Checkpoints, Cell Cycle Proteins/genetics, Chaperonin Containing TCP-1/metabolism, Chromatin/metabolism, Chromosomal Proteins, Non-Histone/metabolism, DNA Damage/genetics, DNA-Binding Proteins/metabolism, Genomic Instability, HeLa Cells, Heat-Shock Proteins/metabolism, Humans, Mutation, Phosphoprotein Phosphatases/genetics, Proteasome Endopeptidase Complex, Protein Folding, Proteins/metabolism, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins/genetics, Sumoylation, Ubiquitination",
author = "Irene Gallina and Camilla Colding and Peter Henriksen and Petra Beli and Kyosuke Nakamura and Judith Offman and Mathiasen, {David P} and Sonia Silva and Eva Hoffmann and Anja Groth and Chunaram Choudhary and Michael Lisby",
year = "2015",
month = mar,
day = "30",
doi = "10.1038/ncomms7533",
language = "English",
volume = "6",
pages = "6533",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control

AU - Gallina, Irene

AU - Colding, Camilla

AU - Henriksen, Peter

AU - Beli, Petra

AU - Nakamura, Kyosuke

AU - Offman, Judith

AU - Mathiasen, David P

AU - Silva, Sonia

AU - Hoffmann, Eva

AU - Groth, Anja

AU - Choudhary, Chunaram

AU - Lisby, Michael

PY - 2015/3/30

Y1 - 2015/3/30

N2 - DNA replication stress is a source of genomic instability. Here we identify changed mutation rate 1 (Cmr1) as a factor involved in the response to DNA replication stress in Saccharomyces cerevisiae and show that Cmr1--together with Mrc1/Claspin, Pph3, the chaperonin containing TCP1 (CCT) and 25 other proteins--define a novel intranuclear quality control compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated proteins in response to genotoxic stress. The diversity of proteins that localize to INQ indicates that other biological processes such as cell cycle progression, chromatin and mitotic spindle organization may also be regulated through INQ. Similar to Cmr1, its human orthologue WDR76 responds to proteasome inhibition and DNA damage by relocalizing to nuclear foci and physically associating with CCT, suggesting an evolutionarily conserved biological function. We propose that Cmr1/WDR76 plays a role in the recovery from genotoxic stress through regulation of the turnover of sumoylated and phosphorylated proteins.

AB - DNA replication stress is a source of genomic instability. Here we identify changed mutation rate 1 (Cmr1) as a factor involved in the response to DNA replication stress in Saccharomyces cerevisiae and show that Cmr1--together with Mrc1/Claspin, Pph3, the chaperonin containing TCP1 (CCT) and 25 other proteins--define a novel intranuclear quality control compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated proteins in response to genotoxic stress. The diversity of proteins that localize to INQ indicates that other biological processes such as cell cycle progression, chromatin and mitotic spindle organization may also be regulated through INQ. Similar to Cmr1, its human orthologue WDR76 responds to proteasome inhibition and DNA damage by relocalizing to nuclear foci and physically associating with CCT, suggesting an evolutionarily conserved biological function. We propose that Cmr1/WDR76 plays a role in the recovery from genotoxic stress through regulation of the turnover of sumoylated and phosphorylated proteins.

KW - Amino Acid Transport Systems/metabolism

KW - Cell Cycle Checkpoints

KW - Cell Cycle Proteins/genetics

KW - Chaperonin Containing TCP-1/metabolism

KW - Chromatin/metabolism

KW - Chromosomal Proteins, Non-Histone/metabolism

KW - DNA Damage/genetics

KW - DNA-Binding Proteins/metabolism

KW - Genomic Instability

KW - HeLa Cells

KW - Heat-Shock Proteins/metabolism

KW - Humans

KW - Mutation

KW - Phosphoprotein Phosphatases/genetics

KW - Proteasome Endopeptidase Complex

KW - Protein Folding

KW - Proteins/metabolism

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins/genetics

KW - Sumoylation

KW - Ubiquitination

U2 - 10.1038/ncomms7533

DO - 10.1038/ncomms7533

M3 - Journal article

C2 - 25817432

VL - 6

SP - 6533

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

ER -

ID: 54999986