Research
Print page Print page
Switch language
Bispebjerg Hospital - a part of Copenhagen University Hospital
Published

Cellular homeostatic tension and force transmission measured in human engineered tendon

Research output: Contribution to journalJournal articlepeer-review

  1. Day-to-Day Reliability of Nonlinear Methods to Assess Walking Dynamics

    Research output: Contribution to journalJournal articlepeer-review

  2. Day-to-day reliability of gait characteristics in rats

    Research output: Contribution to journalJournal articlepeer-review

  1. Collagen Growth Pattern in Human Articular Cartilage of the Knee

    Research output: Contribution to journalJournal articlepeer-review

  2. Mechanical properties of human patellar tendon collagen fibrils. An exploratory study of aging and sex

    Research output: Contribution to journalJournal articlepeer-review

  3. A systematic review of imaging findings in patients with Osgood-Schlatter disease

    Research output: Contribution to journalReviewpeer-review

View graph of relations

Tendons transmit contractile muscular force to bone to produce movement, and it is believed cells can generate endogenous forces on the extracellular matrix to maintain tissue homeostasis. However, little is known about the direct mechanical measurement of cell-matrix interaction in cell-generated human tendon constructs. In this study we examined if cell-generated force could be detected and quantified in engineered human tendon constructs, and if glycosaminoglycans (GAGs) contribute to tendon force transmission. Following de-tensioning of the tendon constructs it was possible to quantify an endogenous re-tensioning. Further, it was demonstrated that the endogenous re-tensioning response was markedly blunted after interference with the cytoskeleton (inhibiting non-muscle myosin-dependent cell contraction by blebbistatin), which confirmed that re-tensioning was cell generated. When the constructs were elongated and held at a constant length a stress relaxation response was quantified, and removing 27% of the GAG content of tendon did not alter the relaxation behavior, which indicates that GAGs do not play a meaningful role in force transmission within this system.

Original languageEnglish
JournalJournal of Biomechanics
Volume78
Pages (from-to)161-165
Number of pages5
ISSN0021-9290
DOIs
Publication statusPublished - 10 Sep 2018

ID: 56198902