Print page Print page
Switch language
Bispebjerg Hospital - a part of Copenhagen University Hospital

Altered Structural Expression and Enzymatic Activity Parameters in Quiescent Ulcerative Colitis: Are These Potential Normalization Criteria?

Research output: Contribution to journalJournal articleResearchpeer-review


  1. Effects of Ketone Bodies on Brain Metabolism and Function in Neurodegenerative Diseases

    Research output: Contribution to journalReviewpeer-review

  2. Matrix Metalloproteinases: How Much Can They Do?

    Research output: Contribution to journalEditorialpeer-review

  3. Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. The Effects of Dual GLP-1/GIP Receptor Agonism on Glucagon Secretion - A Review

    Research output: Contribution to journalReviewpeer-review

  5. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Indications and diagnostic yield of small-bowel capsule endoscopy in a real-world setting

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Glucagon-Like Peptide-2 Analogue ZP1849 Augments Colonic Anastomotic Wound Healing

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Sebastian Kjærgaard
  • Morten M B Damm
  • Joan Chang
  • Lene B Riis
  • Hanne B Rasmussen
  • Rasmus Hytting-Andreasen
  • Susanne M Krug
  • Jörg-Dieter Schulzke
  • Niels Bindslev
  • Mark Berner Hansen
View graph of relations

Mucosal healing determined by endoscopy is currently the remission standard for ulcerative colitis (UC). However, new criteria for remission are emerging, such as histologic normalization, which appears to correlate better to the risk of relapse. Here, we study mucosal healing on a molecular and functional level in quiescent UC. We obtained endoscopic biopsies from 33 quiescent UC patients and from 17 controls. Histology was assessed using Geboes score. Protein and mRNA levels were evaluated for the tight junction proteins claudin-2, claudin-4, occludin, and tricellulin, as well as Cl-/HCO3- exchanger DRA, and cyclo-oxygenase enzymes (COX-1, COX-2). The mucosal activity of COX-1 and COX-2 enzymes was assessed in modified Ussing chambers, measuring electrogenic ion transport (short-circuit current, SCC). Chronic inflammation was present in most UC patients. The protein level of claudin-4 was reduced, while mRNA-levels of claudin-2 and claudin-4 were upregulated in UC patients. Surprisingly, the mRNA level of COX-1 was downregulated, but was unaltered for COX-2. Basal ion transport was not affected, while COX-2 inhibition induced a two-fold larger decrease in SCC in UC patients. Despite being in clinical and endoscopic remission, quiescent UC patients demonstrated abnormal mucosal barrier properties at the molecular and functional level. Further exploration of mucosal molecular signature for revision of current remission standards should be considered.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Issue number5
Publication statusPublished - 10 Mar 2020

    Research areas

  • Adult, Aged, Biopsy, Case-Control Studies, Claudin-1/genetics, Claudins/genetics, Colitis, Ulcerative/genetics, Cyclooxygenase 1/genetics, Cyclooxygenase 2/genetics, Female, Gene Expression Regulation, Gene Regulatory Networks, Humans, MARVEL Domain Containing 2 Protein/genetics, Male, Middle Aged, Young Adult

ID: 62408280